-
Plasmid#40271DepositorInsertClta (Clta Rat)
UseTagsmCitrineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-scFv
Plasmid#78900PurposeExpresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cellsDepositorInsertscFV-VP64
UseCRISPRTagsGFPExpressionInsectMutationPromoterpActin (Drosophila)Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pRK-flag-USP19 1-493
Plasmid#78581PurposeExpression flag-USP19 1-493 in mammalian cellsDepositorInsertflag-USP19 1-493 (USP19 Human)
UseTagsflagExpressionMammalianMutationmutated 494 residue to stop codonPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-link/link-C
Plasmid#61965PurposeNegative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)DepositorInsertsN-mCherry-link
link-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-hFX-promotor(2,5kb)
Plasmid#14980DepositorInserthuman frataxin promoter fragment (FXN Human)
UseLuciferaseTagsExpressionMutationstart codon for human frataxin gene has been muta…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
A3A-Gam
Plasmid#119769PurposeConversion of C to T monocotyledons protoplastsDepositorInsertGam-APOBEC3A-nCas9-NLS-2XUGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISH-Dlx5
Plasmid#74344PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx5. Not for gene expression.DepositorInsertdistal-less homeobox 5 (Dlx5 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Actin-7
Plasmid#55462PurposeLocalization: Actin, Excitation: 462, Emission: 492DepositorInsertActin (ACTB Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IP3KC
Plasmid#32527DepositorInsertIP3KC (ITPKC Human)
UseTags6xHISExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRAN334
Plasmid#120814PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)DepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-C. thermophilum LIC
Plasmid#74780Purposeencodes GST-tagged dynein light intermediate chain from Chaetomium thermophilumDepositorInsertdynein light intermediate chain (Chaetomium thermophilum)
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-hFX-promotor(1,3kb)
Plasmid#14979DepositorInserthuman frataxin promoter fragment (FXN Human)
UseLuciferaseTagsExpressionMutationstart codon for human frataxin gene has been muta…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
Ad-shMKP-3
Plasmid#131530PurposeSilence the MKP-3 expressionDepositorInsertShort hairpin mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationPromoterAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
-
pGEX-6p-1 Sesn1b
Plasmid#61874Purposebacterial expression of a GST-Sesn1b (mouse)DepositorInsertSesn1 (Sesn1 Mouse)
UseTagsGSTExpressionBacterialMutationnonePromoterAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAct:MS2-p65-HSF
Plasmid#78902PurposeExpresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertMS2-p65-HSF
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-cul4
Plasmid#80353PurposePmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjectionDepositorInsertPmex-5:GFP::H2B::PEST::cul-4 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C
Plasmid#61966PurposePositive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))DepositorInsertsN-mCherry-ME(pS)VD
TRAP(RK)-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only