Addgene

We narrowed to 5,233 results for: pCas

Showing: 1781 - 1800 of 5233 results

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pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

Plasmid

#148859

Purpose

Mammalian Expression of HsXRN1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsXRN1-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg1HsAGO2_AH

Plasmid

#148848

Purpose

Mammalian Expression of HsAGO2-sgRNA1

Depositor

Elisa Izaurralde

Insert

HsAGO2-sgRNA1 (AGO2 S.pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6A_AH

Plasmid

#148849

Purpose

Mammalian Expression of HsTNRC6A-sgRNA1

Depositor

Elisa Izaurralde

Insert

HsTNRC6A-sgRNA1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6B_AH

Plasmid

#148850

Purpose

Mammalian Expression of HsTNRC6B-sgRNA1

Depositor

Elisa Izaurralde

Insert

HsTNRC6B-sgRNA1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg2HsAGO2_AH

Plasmid

#148851

Purpose

Mammalian Expression of HsAGO2-sgRNA2

Depositor

Elisa Izaurralde

Insert

HsAGO2-sgRNA2 (AGO2 S.pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg2HsTNRC6A_AH

Plasmid

#148852

Purpose

Mammalian Expression of HsTNRC6A-sgRNA2

Depositor

Elisa Izaurralde

Insert

HsTNRC6A-sgRNA2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg3HsTNRC6B_AH

Plasmid

#148853

Purpose

Mammalian Expression of HsTNRC6B-sgRNA3

Depositor

Elisa Izaurralde

Insert

HsTNRC6B-sgRNA3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH

Plasmid

#148854

Purpose

Mammalian Expression of HsAGO1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsAGO1-sgRNA (AGO1 S.pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsNot1_AH

Plasmid

#148856

Purpose

Mammalian Expression of HsNot1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsNot1-sgRNA (CNOT1 S.pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

Plasmid

#148857

Purpose

Mammalian Expression of HsPatL1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsPatL1-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

Plasmid

#148858

Purpose

Mammalian Expression of HsTNRc6C-sgRNA

Depositor

Elisa Izaurralde

Insert

HsTNRc6C-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAS-mak10 gRNA-MAK10Δ

Plasmid

#160384

Purpose

For CRISPR-mediated deletin og MAK10 in yeast

Depositor

John McCusker

Insert

MAK10

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ8-CadR-Pcadh-LacZ

Plasmid

#191623

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadR

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Tesmin-BioID2-3xFLAG

Plasmid

#186815

Purpose

CAG promoter-driven expression of TESMIN-BioID2-3xFLAG

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Insert

Tesmin (Tesmin Mouse)

Use

Tags

BioID2-3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9-Q835A

Plasmid

#188081

Purpose

Expresses human ATG9-Q835A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

Q835A

Promoter

CMV

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9-K838A

Plasmid

#188078

Purpose

Expresses human ATG9-K838A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

K838A

Promoter

CMV

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-ATG9-V839A

Plasmid

#188077

Purpose

Expresses human ATG9-V839A mutant in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

V839A

Promoter

CMV

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato-2A-mSyp::EGFP

Plasmid

#191213

Purpose

Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

Depositor

Theofanis Karayannis

Article

Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

Insert

tdTomato-T2A-mSyp::EGFP (Syp Mouse)

Use

Pcagen

Tags

mSyp::EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2d (FRRG/AAAA)-TS

Plasmid

#190037

Purpose

Expression of wipi2d mutant

Depositor

James Hurley

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

WIPI2d (WIPI2 Human)

Use

Tags

TEV-2xStrep tag

Expression

Mammalian

Mutation

223FRRG to AAAA

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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2xNLS-NLP-cMyc AspCas12a

Plasmid

#182120

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc AspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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