Addgene

We narrowed to 11,119 results for: 110

Showing: 1781 - 1800 of 11119 results

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pAAV-CBA-Con/Fon-EYFP-WPRE

Plasmid

#186752

Purpose

AAV-mediated Cre and Flp-dependent expression of EYFP under CBA promoter

Depositor

Yongling Zhu

Article

Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

Insert

EYFP

Use

AAV

Tags

Expression

Mutation

Promoter

CBA

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pNB101

Plasmid

#169110

Purpose

Feedback-controlled AraC circuit

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

araC (araC Escherichia coli K12 MG1655)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RhoJQ79L-deltaCaax

Plasmid

#176110

Purpose

Constitutively active, nucelar localized RhoJ

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RhoJ (RHOJ Human)

Use

Tags

Expression

Mammalian

Mutation

Q79L

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA-pHG165c

Plasmid

#170110

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Opie2-PuroR[dsxM]

Plasmid

#131616

Purpose

Expresses a functional PuroR only in males and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR[dsxM] (dsx Fly)
dsRed

Use

Tags

Expression

Insect

Mutation

Promoter

Opie2 and hr5Ie1

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD-H29A-R32A-R36A

Plasmid

#127786

Purpose

Expresses a SsCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

mutations in His29, Arg32 and Arg36 to Ala

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[DsxF].1026A

Plasmid

#112690

Purpose

express gRNA targeting DsxF under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[DsxF] (dsx Fly)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PsuACR_353-EYFP

Plasmid

#111076

Purpose

Anion channelrhodopsin from Proteomonas sulcata (CCMP704) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin PsuACR_353

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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GTT-c

Plasmid

#81108

Purpose

Gene Tagging vector TRP/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

yemCFP

Use

Cre/Lox

Tags

yemCFP

Expression

Yeast

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA27_no stop

Plasmid

#83860

Purpose

Gateway donor vector for IAA27 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA27 (PAP2 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

no stop codon

Promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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KHLH31 pEGFP-N2 (1089)

Plasmid

#62032

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

KHLH31

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pHL1853

Plasmid

#53040

Purpose

PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
tetR
csrA

Use

Synthetic Biology

Tags

LVA, RBS(st2), RBS(st3), and glgC leader

Expression

Bacterial

Mutation

Promoter

pConNoHind and pLlac0-1

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pHL1670

Plasmid

#37634

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
TetR
CsrA (csrA )

Use

Synthetic Biology

Tags

ASV degradation tag and glgC leader region

Expression

Bacterial

Mutation

Promoter

pConM12NoHind and pConNoHind

Available since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only
pHL1668

Plasmid

#37633

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
TetR
CsrA (csrA )

Use

Synthetic Biology

Tags

ASV degradation tag and glgC leader region

Expression

Bacterial

Mutation

Promoter

pConM12NoHind and pConNoHind

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pHL1530

Plasmid

#37569

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
TetR
CsrA

Use

Synthetic Biology

Tags

glgC leader region

Expression

Bacterial

Mutation

start codon removed

Promoter

pConNoHind and pLlacO-1

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pHL601

Plasmid

#37564

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

CsrC (csrC E. coli)
CsrA (csrA E. coli)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pHL1355

Plasmid

#37566

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
TetR
CsrA

Use

Synthetic Biology

Tags

glgC leader region

Expression

Bacterial

Mutation

start codon removed

Promoter

pConNoHind and pConNoHindM12

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only
pHRm-NLS-dCas9-GFP11x7-NLS

Plasmid

#70224

Purpose

Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

NLS-dCas9-GFP11x7-NLS

Use

Tags

GFP11x7

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-PE2max-BSD

Plasmid

#191102

Purpose

Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

PEmax-P2A-BSD

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRK172-6xHis-0N4R-P301S-tau-HiBiT

Plasmid

#187835

Purpose

Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

6XHis-0N4R-P301S-tau-HiBiT

Use

Tags

6xHistidine and HiBiT

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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