Addgene

We narrowed to 8,107 results for: sgRNA

Showing: 161 - 180 of 8107 results

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p183_LTJ_sgRNACD90.2_A

Plasmid

#82671

Purpose

sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD90.2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJT303_SNR52_sgRNA_Can1-3

Plasmid

#145066

Purpose

Expresses sgRNA targeting Can1-3 site

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

Can1-3 sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_PSMD1_sgRNA_1

Plasmid

#155107

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting PSMD1 (core essential gene)

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

PSMD1_sgRNA_1 (PSMD1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_AAVS1_sgRNA_2

Plasmid

#155088

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_CELF2

Plasmid

#106102

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting CELF2 (CELF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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EMX1_sgRNA

Plasmid

#100555

Purpose

Expresses EMX1 sgRNA. Target sequence: GAGTCCGAGCAGAAGAAGAA

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

EMX1 sgRNA

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_hSt1Cas9_CNRZ1066

Plasmid

#136651

Purpose

A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA.

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9:CNRZ_1066 Chimera
sgRNA for St1Cas9

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG and hU6

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMB2_sgRNA_5

Plasmid

#74185

Purpose

lentiviral vector expressing sgRNA targeting PSMB2

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMB2 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_RNF138_G1

Plasmid

#127120

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA RNF138 (RNF138 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_RNF123

Plasmid

#127122

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA RNF123 (RNF123 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_CELF1

Plasmid

#106101

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting CELF1 (CELF1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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sgRNA4_NANOG

Plasmid

#64156

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant

Plasmid

#106112

Purpose

Inducible gene expression of FlagHA-PRKRA sgRNA PAM mutant

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

silent mutation of Gly32 (GGG-->GGT)

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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sgRNA3_NANOG

Plasmid

#64155

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA3_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA2_NANOG

Plasmid

#64154

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA2_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBSsgRNA

Plasmid

#224867

Purpose

Yeast genome editing, Insertion of 20 bp oligo into sgRNA gene

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA TR KO 2

Plasmid

#207610

Purpose

sgRNA 2 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

TCAGGCCGCAGGAAGAGGAA

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA TR KO 1

Plasmid

#207609

Purpose

sgRNA 1 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

ACCCTAACTGAGAAGGGCGT

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSGKP_AcsgRNA

Plasmid

#203807

Purpose

pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23119

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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GLE_sgRNA

Plasmid

#186658

Purpose

GLE sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Gle sgRNA Plasmid (CG14749 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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