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Plasmid#105344PurposeYeast two hybridDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only
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psbA2-PHLS (b)
Plasmid#52307PurposeReplaces the psbA2 gene in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpsbA2Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMBA326
Plasmid#214743PurposeHigh copy glmS-nadE plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSV014
Plasmid#214740PurposeHigh copy thyA-infA plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXC3cIfm
Plasmid#193750PurposeExpresses frame-shifted CI under the control of PLUX promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertFrame-shifted CI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLUXAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO5
Plasmid#193749PurposeExpresses tdTomato under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selectionDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterPIAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO3
Plasmid#193748PurposeExpresses E2-Crimson under the control of PAAH synthetic promoter regulated by AHL and aTc, p15A origin of replication, Chloramphenicol selectionDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPAAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO6
Plasmid#193747PurposeExpresses E2-Crimson under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selection,DepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPIAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX*C3E2-Crimson
Plasmid#193754PurposeExpresses E2-Crimson under the control of PLUX* promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLUX*Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term SMT3 NikJC199U
Plasmid#174210PurposeExpression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term SMT3 NikJC199U
Plasmid#174362PurposeExpression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, SMT3, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm N-term MBP NikJC199U
Plasmid#174364PurposeExpression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, and TEVExpressionBacterialMutationPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term MBP NikJC199U
Plasmid#174365PurposeExpression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
UseTags8xHis, MBP, and TEVExpressionBacterialMutationC199UPromoterT7Available sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOG131
Plasmid#105337PurposeGateway entry clone generationDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG267
Plasmid#105339PurposeSynthetic biology; gateway destination vector assemblyDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
ARK2a
Plasmid#81017PurposeE.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco
PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARK1a
Plasmid#81016PurposeE.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-PMKco
PMD-NudB
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJH123
Plasmid#68379PurposepJH122 variant Gateway destinationDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJH145
Plasmid#68380PurposeIMCE attB donor copy control fosmidDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pminTolR4-R2
Plasmid#67711Purposemini Tol2 backbone with att4-attR2 cassetteDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only