1. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. lenti-SpCas9 puro

   Plasmid

   #104994

   Purpose

   This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiCRISPRv2 puro

   Plasmid

   #98290

   Purpose

   Variant of lentiCRISPRv2 that confers puromycin resistance

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiCRISPRv2 neo

   Plasmid

   #98292

   Purpose

   Variant of lentiCRISPRv2 that confers G418 resistance

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lenti-SpCas9 neo

   Plasmid

   #104996

   Purpose

   This lentiviral construct delivers hSpCas9 and G418 resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lenti-SpCas9 hygro

   Plasmid

   #104995

   Purpose

   This lentiviral construct delivers hSpCas9 and hygromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lenti-SpCas9 blast

   Plasmid

   #104997

   Purpose

   This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lentiCRISPR v2-Nr4a1 double gRNAs

   Plasmid

   #162791

   Purpose

   Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiCRISPR v2 kif26b sgRNA1

   Plasmid

   #102857

   Purpose

   Expresses sgRNA targeting mouse Kif26b

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   Mouse Kif26b (Kif26b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiCRISPR v2 kif26b sgRNA2

   Plasmid

   #102858

   Purpose

   Expresses sgRNA targeting mouse Kif26b

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   Mouse Kif26b (Kif26b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LCV2_Luciferase_sgRNA

    Plasmid

    #155094

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting Luciferase

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    Luciferase_sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GluR1-Flop/pRK5

    Plasmid

    #197308

    Purpose

    Plasmid expressing an antigen targeting the GluR1/GluA1 AMPA receptor subunit

    Depositor

    Robert Wenthold

    Insert

    GluR1-Flop (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSIN ReP5-GFP-GluR1

    Plasmid

    #24004

    Depositor

    Robert Malinow

    Article

    Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

    Insert

    GluR1 (Gria1 Rat)
    

    Use

    Sindbis virus production

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pTRE_GFP-GluR1

    Plasmid

    #34857

    Depositor

    Mark Mayford

    Article

    Matsuo et al Science. 2008 Feb 22;319(5866):1104-7.

    Insert

    GluR1 (Gria1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-SEP-GluR1

    Plasmid

    #24000

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    GluR1 (Gria1 Rat)
    

    Use

    Tags

    GluR1 signal peptide (aa1-22) and SEP (supereclip…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFPC1-GluR1Ctail

    Plasmid

    #32441

    Depositor

    Robert Malinow

    Article

    Rumpel et al Science. 2005 Apr 1;308(5718):83-8. Epub

    Insert

    GluR1 (GRIA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains the last C-terminal 81 amino acids of th…

    Promoter

    CMV

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQTEV-NOHMA

    Plasmid

    #34821

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    HORMA domain containing protein (HORMAD1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFlc245

    Plasmid

    #101021

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFlg167

    Plasmid

    #101019

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    cmv

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFlg113

    Plasmid

    #101020

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    cmv

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFlg262

    Plasmid

    #101016

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge