168,579 results

1. pCK029 exorh:EGFP

   Plasmid

   #195980

   Purpose

   Expression construct

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   exorh:EGFP
   

   Use

   Other

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Puro-miRFP670nano3

   Plasmid

   #207691

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miRFP670nano3 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

   Plasmid

   #134923

   Purpose

   Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUTKan-3xHA-GFP-TOM5

   Plasmid

   #130674

   Purpose

   Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

   Depositor

   Andreas Weber

   Article

   Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

   Insert

   Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)
   

   Tags

   3xHA and sGFP

   Expression

   Plant

   Promoter

   Ubiquitin10

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBadcrtEBI_13

   Plasmid

   #63811

   Purpose

   expresses crtEBI genes for neurosporene production

   Depositor

   Howard Salis

   Article

   Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

   Insert

   crtEBI operon
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   PBAD

   Available Since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKL2013

   Plasmid

   #172182

   Purpose

   A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.

   Depositor

   Kan Wang

   Article

   McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d

   Insert

   mCherry
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN-PITCh-mCherry

   Plasmid

   #127887

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter mCherry tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   Puro-mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2_claudink:Cas9green; control_gRNA

   Plasmid

   #199336

   Purpose

   pDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   claudink
   Cas9
   control sgRNA
   

   Use

   Tol2 destination vector

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG426GPD-EGFP-ccdB

   Plasmid

   #14324

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_DNTTIP2_WT_V5

    Plasmid

    #82939

    Purpose

    Gateway Donor vector containing DNTTIP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    DNTTIP2 (DNTTIP2 Human)
    

    Use

    Gateway entry vector

    Mutation

    E309D

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. BL21 Rosetta2 ΔrecB

    Bacterial Strain

    #176582

    Purpose

    E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

    Depositor

    Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    Chloramphenicol

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

12. pET11a-p65(19-325)

    Plasmid

    #44744

    Depositor

    Gourisankar Ghosh

    Insert

    p65 (Rela Mouse)
    

    Expression

    Bacterial

    Mutation

    AA 19-325

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

13. p1153

    Plasmid

    #222930

    Purpose

    Shuttle vector carrying a Candida auris centromere (CEN 4) with Nourseothricin selectable marker

    Depositor

    Brian Wickes

    Article

    Determann B et al J Fungi (Basel). 2024 Jul 11;10(7):477.

    Insert

    CEN 4
    

    Expression

    Bacterial

    Available Since

    July 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAG416GPD-EGFP-ccdB

    Plasmid

    #14316

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. EYFP-NIK

    Plasmid

    #111208

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

    Insert

    NIK (MAP3K14 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. LSB-hsa-miR-150-5p

    Plasmid

    #103257

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-150-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-150-5p target (MIR150 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET11a-p52(1-398)

    Plasmid

    #44733

    Depositor

    Gourisankar Ghosh

    Insert

    p52 (NFKB2 Human)
    

    Expression

    Bacterial

    Mutation

    AA 1-398

    Promoter

    T7

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

18. LSB-hsa-miR-23a-3p

    Plasmid

    #103371

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-23a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-23a-3p target (MIR23A Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PHYHD1

    Plasmid

    #38854

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PHYHD1 (Phyhd1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pTol1-U6abc_ect2_cmlc2-nmKate

    Plasmid

    #238410

    Purpose

    Drives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytes

    Depositor

    Juan Manuel González-Rosa

    Article

    Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

    Insert

    nuclear mKate/3 gRNAs targeting ect2
    

    Promoter

    cmlc2 (nmKate); U6 (gRNAs)

    Available Since

    Nov. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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