169,099 results

1. mCherry-cGAS-HA

   Plasmid

   #231895

   Purpose

   Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   cGAS (CGAS Human)
   

   Tags

   3xHA and mCherry

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVdual-CMV-eGFP

   Plasmid

   #230931

   Purpose

   pAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter.

   Depositor

   Qizhao Wang

   Article

   Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

   Insert

   eGFP
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCS-BioID2-HA

   Plasmid

   #74224

   Purpose

   To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   HA and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST_FLAG-HUWE1

   Plasmid

   #187120

   Purpose

   mammalian cell expression of FLAG-HUWE1

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Tags

   1xFLAG

   Expression

   Mammalian

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hsyn-GRAB_DA2h (AAV9)

   Viral Prep

   #140554-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA2h (#140554). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA2h plasmid DNA. Synapsin-driven expression of high-affinity GRAB_DA2h dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Promoter

   Syn

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pCS2+IMS-HyPer7

   Plasmid

   #136469

   Purpose

   Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   SMAC/DIABLO

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLAMP1-mCherry

   Plasmid

   #45147

   Depositor

   Amy Palmer

   Article

   Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

   Insert

   LAMP1-mCherry (LAMP1 Human, Synthetic)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAL-7xHis-TEV-hexa

   Plasmid

   #222868

   Purpose

   Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

   Depositor

   Nikolaus Loening

   Article

   Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

   Insert

   TEV protease
   

   Tags

   7xHis and MBP

   Expression

   Bacterial

   Mutation

   C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJRH-1179 U6-reci Gag-Cas9 v2

   Plasmid

   #201914

   Purpose

   Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Gag-Cas9 v2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBKT7-GW

    Plasmid

    #61703

    Purpose

    Gateway compatible Y2H vector - BD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGADT7-GW

    Plasmid

    #61702

    Purpose

    Gateway compatible Y2H vector - AD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJRH-1180 U6-reci Gag-pol v2

    Plasmid

    #201915

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-pol
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYJA5

    Plasmid

    #217778

    Purpose

    The empty vector for quadruple sgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Puromycin resistance gene, T2A, and TagBFP

    Expression

    Mammalian

    Promoter

    PGK, CMV, U6

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV9)

    Viral Prep

    #100833-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pHL-sec

    Plasmid

    #99845

    Purpose

    Mammalian expression with secretion signal sequence

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EFNB2 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-hM4D(Gi)-mCherry

    Plasmid

    #50475

    Purpose

    Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pM-CBE

    Plasmid

    #196293

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding CBE in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGTR

    Plasmid

    #63143

    Purpose

    PCR template to construct polycistronic tRNA-gRNA

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    gRNA-tRNA fusion
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

    Plasmid

    #101060

    Purpose

    AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-UltraCas12a-6xNLS

    Plasmid

    #209023

    Purpose

    Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    UltraCas12a-6xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    M537R/F870L

    Promoter

    EF-1a

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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