320,890 results

1. pCS2FA CO Tol2 TPase

   Plasmid

   #133032

   Purpose

   Expression vector for production of codon optimized Tol2 transposase

   Depositor

   Charles Nathan Hancock

   Article

   Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

   Insert

   Codon optimized Tol2 Transposase
   

   Use

   In vitro expression

   Mutation

   Codon Optimized

   Available Since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2FA CO Tol2 TPase

   Plasmid

   #133032

   Purpose

   Expression vector for production of codon optimized Tol2 transposase

   Depositor

   Charles Nathan Hancock

   Article

   Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

   Insert

   Codon optimized Tol2 Transposase
   

   Use

   In vitro expression

   Mutation

   Codon Optimized

   Available Since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3/Myc-DNMT1

   Plasmid

   #36939

   Purpose

   Mammalian expression of human DNMT1 with myc tag

   Depositor

   Arthur Riggs

   Article

   Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

   Insert

   DNMT1 (DNMT1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3/Myc-DNMT1

   Plasmid

   #36939

   Purpose

   Mammalian expression of human DNMT1 with myc tag

   Depositor

   Arthur Riggs

   Article

   Li et al J Biol Chem. 2006 Jul 14;281(28):19489-5

   Insert

   DNMT1 (DNMT1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

   Plasmid

   #139999

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

   Plasmid

   #139999

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti PGK Neo DEST (w531-1)

   Plasmid

   #19067

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti PGK Neo DEST (w531-1)

   Plasmid

   #19067

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9_T2A_iRFP670 sgAAVS1

   Plasmid

   #192477

   Purpose

   All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9_T2A_iRFP670 sgAAVS1

    Plasmid

    #192477

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-EF1a-dCas9-VPR-Puro

    Plasmid

    #99373

    Purpose

    3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    (Sp)dCas9-VPR-P2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCRISPRv2-STING_gRNA3

    Plasmid

    #127640

    Purpose

    Knock-out of human STING

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCRISPRv2-STING_gRNA3

    Plasmid

    #127640

    Purpose

    Knock-out of human STING

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-mCherry

    Plasmid

    #128744

    Purpose

    Expresses mCherry in mammalian cells

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-mCherry

    Plasmid

    #128744

    Purpose

    Expresses mCherry in mammalian cells

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GRAB-rDA3m

    Plasmid

    #208702

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-GRAB-rDA3m

    Plasmid

    #208702

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (AAV8)

    Viral Prep

    #154868-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ulrik Gether

    Promoter

    Human Synapsin-1

    Tags

    mCherry (Flp-dependent)

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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