168,547 results

1. Stargazin-GFP-LOVpep

   Plasmid

   #80406

   Purpose

   Membrane localized GFP-tagged LOVpep. Expresses in mammalian cells

   Depositor

   Michael Glotzer

   Article

   Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

   Insert

   Stargazin-GFP-LOVpep (CACNG2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUltraHot-mCherry-DHX36-iso1-WT

   Plasmid

   #206964

   Purpose

   expressing the protein DHX36 fused to mCherry in human cells

   Depositor

   Eran Hornstein

   Article

   Danino et al Nucleic Acids Res. 2023 Jul 28:gkad613.

   Insert

   DEAH-Box Helicase 36 (DHX36 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSicoR-mCh-empty

   Plasmid

   #21907

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

   Depositor

   Miguel Ramalho-Santos

   Article

   Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1-His10FLAG

   Plasmid

   #157736

   Purpose

   empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

   Depositor

   Debra Hansen

   Article

   Olson et al Protein Expr Purif. 2021 May 7:105890. d

   Type

   Empty backbone

   Tags

   His10 + 3xFLAG, enterokinase-cleavable

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PR_GEF (2XPDZ-mCherry-Larg (DH)

   Plasmid

   #80407

   Purpose

   Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

   Depositor

   Michael Glotzer

   Article

   Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

   Insert

   2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   DH domain only (aa 766-997)

   Promoter

   CMV

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYLsgRNA-AtU3b/LacZ

   Plasmid

   #66199

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   AtU3b

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. DD-DamV133A-tdTomato-LMNB1

   Plasmid

   #159604

   Purpose

   Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.

   Depositor

   Aaron Streets

   Article

   Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

   Insert

   DD-DamV133A-tdTomato-LMNB1
   

   Expression

   Mammalian

   Mutation

   Dam V133A mutation

   Promoter

   CMV

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYLsgRNA-OsU6a/LacZ

   Plasmid

   #66195

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   OsU6a

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJS169

   Plasmid

   #48882

   Purpose

   Negative feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Insert

   lacI with ssrA tag
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Promoter

   Para/lac

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJS167

    Plasmid

    #48881

    Purpose

    Positive feedback plasmid from oscillator

    Depositor

    Jeff Hasty

    Article

    Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

    Inserts

    araC gene with ssrA tag
    monomeric yeast-enhanced green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    ssrA (TSAANDENYALAA)

    Expression

    Bacterial

    Promoter

    Para/lac

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b-ELP[V]60

    Plasmid

    #219406

    Purpose

    (VPGVG)60

    Depositor

    Danielle Mai

    Article

    Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

    Insert

    ELP[V]60
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. FR_HNF6

    Plasmid

    #31099

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF6 (ONECUT1 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ2072.2_c1

    Plasmid

    #140675

    Purpose

    CRISPRlator control (“open ring”)

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    CRISPRlator control (“open ring”)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRCA892 - TFORF Empty Vector Puro-T2A-GFP (pRCA886 backbone)

    Plasmid

    #238180

    Purpose

    Lentiviral empty vector

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-FOXE3

    Plasmid

    #153121

    Purpose

    Expression of Flag tagged human FOXE3 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXE3 (FOXE3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-C-Solo-mStayGold-COX8A

    Plasmid

    #227309

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-PITCh-COX8A

    Plasmid

    #227308

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of COX8A (COX8A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U67

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV2btCRISPRko.v1

    Pooled Library

    #213927

    Purpose

    Uses lentivirus to deliver the library into hard-to-transduce cells

    Depositor

    Wenfang Spring Tan

    Article

    Tan et al PLoS Pathog. 2023 Dec 6;19(12):e1011822.

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

19. DD-DamV133A-IRES2-mCherry

    Plasmid

    #159600

    Purpose

    Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the V133A mutant of Dam.

    Depositor

    Aaron Streets

    Article

    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

    Insert

    DD-DamV133A-IRES2-mCherry
    

    Expression

    Mammalian

    Mutation

    Dam V133A mutation

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA5/tom20-pbira(79-c)-ha

    Plasmid

    #154456

    Purpose

    expresses tom20-pbira(79-c)-ha/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    tom20-pbira(79-c)-ha (TOMM20 Human)
    

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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