168,841 results

1. pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2

   Plasmid

   #215326

   Purpose

   Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

   Depositor

   Rob Hynds

   Article

   Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

   Insert

   dNTP63 Promoter
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   dNTP63

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

   Plasmid

   #208035

   Purpose

   Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   SUMO1nc (SUMO1 Human)
   

   Use

   Lentiviral

   Tags

   Myc, C-terminal Split-TurboID

   Mutation

   Q94P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTarget12f1

   Plasmid

   #171183

   Purpose

   Constitutive expression of sgRNA without donor DNA

   Depositor

   Kohsuke Honda

   Article

   Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

   Insert

   sgRNA scaffold in the CRISPR/Cas12f1 system
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119(SpeI) promoter

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DIO SwiChRca-TS-EYFP

   Plasmid

   #55631

   Purpose

   Cre-Dependent Chloride Channel

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

   Insert

   iC1C2-TS-EYFP
   

   Use

   AAV and Cre/Lox

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C128A

   Promoter

   Ef1a

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-mRIN2

   Plasmid

   #108940

   Purpose

   Mammalian expression of mRIN2

   Depositor

   Guido Serini

   Article

   Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

   Insert

   Ras and Rab interactor 2 (Rin2 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ265E9

   Plasmid

   #213472

   Purpose

   Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadDE-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tadde-32aa linker-zspc…

   Tags

   NO

   Expression

   Plant

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-secAnxaV-NS

   Plasmid

   #67718

   Purpose

   Gateway entry clone containing secreted human AnxaV with no stop codon

   Depositor

   Rob Parton

   Article

   Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

   Insert

   secAnxaV-NS
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP12609 - pAAV-AiE0779m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2609)

   Plasmid

   #191705

   Purpose

   AI plasmid ALIAS: AiP12609. Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   none

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Circular 200,100 (mPCSK9)

   Plasmid

   #170122

   Purpose

   AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (Pcsk9 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6 and mouse U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA

    Plasmid

    #180590

    Purpose

    Opto-cytTrkB

    Depositor

    Won Do Heo

    Article

    Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

    Insert

    TrkB
    

    Use

    AAV

    Mutation

    PHR(E281A)

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. iRFP-PAmCherry1-PH_PLCd1

    Plasmid

    #92167

    Purpose

    PI(4,5)P2 single molecule fluorescent reporter

    Depositor

    Xuelin Lou

    Article

    Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

    Insert

    iRFP-PAmCherry1-PH_PLCd1
    

    Tags

    iRFP-PAmCherry1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. B*57:03:01

    Plasmid

    #203260

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*57:03:01 (HLA-B Human)
    

    Expression

    Mammalian

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Circular 200,100 (mIDUA)

    Plasmid

    #170123

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Idua Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6 and mouse U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTNR1A-DuET

    Plasmid

    #213348

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MTNR1A (MTNR1A Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. INCENP

    Plasmid

    #39185

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    INCENP (INCENP Human)
    

    Tags

    GST-Tev

    Expression

    Bacterial

    Mutation

    Complex with AURKBA-c004

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pHJ4: LexA-VHH-Caffeine

    Plasmid

    #108242

    Purpose

    LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    LexA DNA binding domain fusing with VHH-Caffeine antibody
    

    Use

    Synthetic Biology

    Promoter

    pLacO1

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hNR2F2-RFP

    Plasmid

    #22926

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1-PHD3

    Plasmid

    #21402

    Depositor

    Eric Metzen

    Article

    Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

    Insert

    EGLN3 (EGLN3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

19. ZNF668-Flag

    Plasmid

    #42636

    Depositor

    Shiaw-Yih Lin

    Article

    Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

    Insert

    ZNF668 (ZNF668 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

20. OLIG2_pCSdest

    Plasmid

    #53858

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    OLIG2 (OLIG2 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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