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Plasmid#74072PurposepAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecRDepositorInsertsgRNA pyk (NT)
UseCRISPRTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDD379
Plasmid#91834PurposeSapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3s-Cas9-HN
Plasmid#104171PurposeCas9-WT mammalian expressionDepositorInsertCas9-WT
UseCRISPRTagsHAExpressionMammalianMutationWTPromoterCMVAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCSh5-2300
Plasmid#171788PurposeEncodes sgRNA target 2300 and Gentamicin resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ283
Plasmid#52224PurposeExpression of sgRNA precursor with CspCI placeholder for target cloning (see comments).DepositorInsertrrk1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_NT
Plasmid#74069PurposepAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecRDepositorInsertsgRNA pck (NT)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pgi_T
Plasmid#74066PurposepAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecRDepositorInsertsgRNA pgi (T)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP2754
Plasmid#160666PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAL-pyk_T
Plasmid#74070PurposepAL374 plasmid carrying the pyk (T) sgRNA targeting the template strand of pyk, SpecRDepositorInsertsgRNA pyk (T)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-pck_T
Plasmid#74068PurposepAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecRDepositorInsertsgRNA pck (T)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-rfp_T
Plasmid#74075PurposepAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecRDepositorInsertsgRNA rfp (T)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS719
Plasmid#188894PurposeminiMos Arm sgRNA expression vectorDepositorInsertPU6(K09B11.12)::miniMos Arm sgRNA
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6>Ebf.774(F+E)
Plasmid#59990PurposeU6 promoter driving Ebf.774 (F+E) sgRNADepositorInsertEbf.774 (F+E) sgRNA
UseCRISPRTagsExpressionMutationPromoterCiinte.U6Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
U6>Control(F+E)
Plasmid#60006PurposeU6 promoter driving control (F+E) sgRNADepositorInsertControl (F+E) sgRNA
UseCRISPRTagsExpressionMutationPromoterCiinte.U6Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMP2480
Plasmid#160080PurposeZ. mobilis CRISPRi, promoter C, empty guideDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWT060e
Plasmid#107905PurposeW2m.0-3, W2m.3-2 plasmid. Contains U6-sgRNA B (CCR5) and is part of the CAMERA2m system for cellular recording in mammalian cellsDepositorInsertU6-sgRNA B (CCR5)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-ERCC6L gRNA2
Plasmid#211633PurposesgRNA-2 agsinst ERCC6LDepositorInsertsgRNA ERCC6L
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-ZWILCH gRNA2
Plasmid#211635PurposesgRNA-2 against ZWILCHDepositorInsertsgRNA ZWILCH
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-ZWILCH gRNA1
Plasmid#211634PurposesgRNA-1 against ZWILCHDepositorInsertsgRNA ZWILCH
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-ERCC6L gRNA1
Plasmid#211632PurposesgRNA-1 against ERCC6LDepositorInsertsgRNA ERCC6L
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only