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We narrowed to 4,925 results for: pcDNA3.1+

Showing: 1761 - 1780 of 4925 results

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pcDNA3.1-FLAG-SBP-STAG2 (S1075X)

Plasmid

#73967

Purpose

STAG2 cDNA (CCDS43990) S1075X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S1075X

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (S653X)

Plasmid

#73965

Purpose

STAG2 cDNA (CCDS43990) S653X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S653X

Promoter

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)

Plasmid

#227614

Purpose

Overexpression of SLC25A46 in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Use

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

Plasmid

#227619

Purpose

Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Use

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

deleted aa 2-83

Promoter

CMV, T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

Plasmid

#222861

Purpose

This plasmid codes for the OgeuIscB nickase

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

OgeuIscB Nickase

Use

CRISPR

Tags

3X HA and Nucleoplasmin NLS and ITR2

Expression

Mammalian

Mutation

E193A for nickase mutation

Promoter

CMV

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT

Plasmid

#215898

Purpose

Expression of TNRC6A-CIM1 domain

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT

Plasmid

#215899

Purpose

Expression of TNRC6A-CIM2 domain

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1529-1709

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(G12C, K96H)

Plasmid

#224281

Purpose

Cell transfection and expression of Rac1 (G12C, K96H)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

G12C, K96H

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rap1A(G12C, L96F)

Plasmid

#224277

Purpose

Cell transfection and expression of Rap1A (G12C, L96F)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rap1A (RAP1A Human)

Use

Tags

Expression

Mammalian

Mutation

G12C, L96F

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-RNF8-C403S

Plasmid

#221685

Purpose

Mammalian expression of Myc-tagged BirA*-RNF8 (C403S) fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Tags

Expression

Mammalian

Mutation

C403S

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-RNF8-R42A

Plasmid

#221684

Purpose

Mammalian expression of Myc-tagged BirA*-RNF8 (R42A) fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Tags

Expression

Mammalian

Mutation

R42A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-HsPcdh21-Myc-Flag-His

Plasmid

#210818

Purpose

Mammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Protocadherin 21 (CDHR1 Human)

Use

Tags

Myc, 3x Flag, 10x His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MF3958 Heavy chain-SpyTag003

Plasmid

#216303

Purpose

Expresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

MF3958 Heavy chain-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pert Heavy chain-SpyTag003

Plasmid

#216309

Purpose

Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

H2-18 Heavy chain-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tras heavy chain-SpyTag003

Plasmid

#216293

Purpose

Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Tras heavy chain-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-39S Heavy chain-SpyTag003

Plasmid

#216296

Purpose

Expresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

39S Heavy chain-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-α-COPI-WD40-Arg13Ala

Plasmid

#208172

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 Arg13Ala mutant (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)

Use

Tags

Strep-tag

Expression

Mammalian

Mutation

Arg13Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-α-COPI-WD40-Arg300Ala

Plasmid

#208173

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg300Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys,Arg300Ala)

Use

Tags

Strep-tag

Expression

Mammalian

Mutation

Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe19…

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-α-COPI-WD40-Lys15Ala

Plasmid

#208174

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Lys15Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

N-terminal WD40 domain of yeast α-COPI(1-327);six substitutions(Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys, Phe197Lys)

Use

Tags

Strep-tag

Expression

Mammalian

Mutation

Lys15Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196…

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-WT

Plasmid

#218460

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues)-wild type (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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