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Plasmid#206272PurposeENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoterDepositorInserteGFP
UseMultimate/gateway entr 4TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 4 CMV mTagBFP
Plasmid#206279PurposeENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.DepositorInsertmTagBFP
UseMultimate/gateway entr 4TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-IEo
Plasmid#201992PurposeAAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virusDepositorInsertInducible expression of codon optimized immediate early gene IE180 of pseudorabies virus
UseAAVTagsExpressionMutationPromoterTight TREAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRANS-Pho2-1/2-2-Csy4
Plasmid#161763PurposepTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)DepositorInsertCas9 and gRNAs
UseTagsExpressionPlantMutationPromoterCmYLCV PromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRANS-Pho2-1/2-2-tRNA
Plasmid#161762PurposepTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)DepositorInsertCas9 and gRNAs
UseTagsExpressionPlantMutationPromoterCmYLCV PromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
iEscSnFR
Plasmid#182807PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR
UseTags6xHis, HA and MycExpressionBacterialMutationPromoterT7Available sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
iFluoxSnFR
Plasmid#182808PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR
UseTags6xHis, HA and MycExpressionBacterialMutationPromoterT7Available sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJH4235
Plasmid#191534PurposePsra-11-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans AVB/others neuron expression of GtACR2 RFPDepositorInsertGtACR2
UseTagswCherryExpressionWormMutationPromoterPsra-11Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4253
Plasmid#191539PurposePrig-3-LoxP::EBFP::LoxP::Chrimson::wCherry unc-54 3' UTR C.elegans AVA neuron expression of Chrimson RFPDepositorInsertChrimson
UseTagswCherryExpressionWormMutationPromoterPrig-3Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH2633
Plasmid#192107PurposePsra-11-TeTx::wCherry unc-54 3' UTR C.elegans AVB and other neurons expression of TeTx RFPDepositorInsertTetanus Toxin Light Chain
UseTagswCherryExpressionWormMutationPromoterPsra-11Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH4217
Plasmid#191533PurposePrig-3-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans AVA neuron expression of GtACR2 RFPDepositorInsertGtACR2
UseTagswCherryExpressionWormMutationPromoterPrig-3Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY80
Plasmid#191075Purposeflp-18(AVA = 4.2-1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-18(AVA = 4.2-1k) (flp-18 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY73
Plasmid#191070Purposecho-1(3.7-1.7k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertcho-1(3.7-1.7k) (cho-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY72
Plasmid#191069Purposecho-1(3.7k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertcho-1(3.7k) (cho-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
Plasmid#182921PurposeExpresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.DepositorInsertMKI67 (MKI67 Human)
UseTagsEGFP and mCherryExpressionBacterial and MammalianMutationRASA mutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMERVK9c_1 (inducible)
Plasmid#189957PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK9c
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMERVK10c_2 (inducible)
Plasmid#189956PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK10c
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMETn_2 (inducible)
Plasmid#189954PurposeshRNA mediated knockdownDepositorInsertERV_MMETn
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMETn_1 (inducible)
Plasmid#189953PurposeshRNA mediated knockdownDepositorInsertERV_MMETn
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only