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Plasmid#51722PurposePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!DepositorInsertSpyLigase
UseTagsHis6 TagExpressionBacterialMutationPromoterT7Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Aqua
Plasmid#42889DepositorInsertAquamarine
UseTags6xHis and TEV cleavageExpressionBacterialMutationT65S and H148GPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFH3.2_mTagBFP2-MS2x12
Plasmid#205558PurposeExpress mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mRuby3
Plasmid#74234PurposeConstitutive expression of mRuby3 in bacteriaDepositorInsertmRuby3
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-8xbiRhoBAST
Plasmid#196351PurposeExpression of mEGFP mRNA with 8xbiRhoBAST in 3'UTRDepositorInsertmEGFP-8xbiRhoBAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPUR-hU6-sgRNA-Sirius-8XMS2
Plasmid#121942PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PCNA-IRES-puro2b
Plasmid#26461DepositorInsertPCNA (PCNA Human)
UseTagspEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
mVenus
Plasmid#198192PurposeSpectral correction: Expression of mVenusDepositorInsertmVenus
UseTagsExpressionMammalianMutationA206KPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG1247 H2B-ECFP-FIREmate
Plasmid#205171PurposeExpresses H2B-ECFP-FIREmate in mammalian cellsDepositorInsertH2B-ECFP-FIREmate
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AncBE4stem
Plasmid#208766PurposeAncBE4max that co-expresses UGI and p53DDDepositorInsertAncBE4max with 2A-UGI and 2A-p53DD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry
Plasmid#34911DepositorInsertpresynaptic mGRASP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-KIRIN1-GR
Plasmid#107418PurposeMammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1-GRDepositorInsertKIRIN1-GR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Set9 wt
Plasmid#24084DepositorInsertSet9 (SETD7 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Orange_CaMBI_110
Plasmid#124094Purposeorange calcium-modulated bioluminescent indicator, 110nMDepositorInsertOrange CaMBI 110
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cox8x2-CeNL_pcDNA3
Plasmid#89513PurposeCyan color variant of bright luminescent protein, targeted to the MitochondriaDepositorInsertCeNL
UseTagsCoxVIII X2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCP-YFP
Plasmid#101160Purposeto express MCP-YFP fusion proteinDepositorInsertMCP-YFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cox8x2-ReNL_pcDNA3
Plasmid#89533PurposeRed color variant of bright luminescent protein, targeted to the MitochondriaDepositorInsertReNL
UseTagsCoxVIII X2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
B-gTEMP/pcDNA3
Plasmid#188447PurposeB-gTEMP for mammalian expressionDepositorInsertB-gTEMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QNA
Plasmid#129305PurposeNegative control for ATP measurementDepositorInsertQUEEN-NA
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-eGFP-MCS-polyA
Plasmid#174024PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInserteGFP
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only