Addgene

We narrowed to 12,947 results for: flag

Showing: 1741 - 1760 of 12947 results

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p3xFLAG-Tmprss6(Mask)

Plasmid

#18790

Depositor

Bruce Beutler

Article

Du et al Science. 2008 May 23. 320(5879):1088-92.

Insert

Type II transmembrane serine protease 6 (Tmprss6 Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 567-811 and replaced with 28 …

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pKK-TEV-3XFLAG

Plasmid

#105784

Purpose

Expression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-FLAGLICNot

Plasmid

#140184

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

FLAG

Expression

Mutation

Promoter

T7

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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Flag SGK3 wt - pCMV

Plasmid

#24650

Depositor

Jim Woodgett

Article

Tessier et al J Biol Chem. 2006 Aug 18. 281(33):23978-

Insert

SGK3 (SGK3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pcdna3 Flag Jip2

Plasmid

#59027

Purpose

act as molecular scaffolds that mediate the activation of the JNK signaling pathway in vivo.

Depositor

Roger Davis

Article

Yasuda et al Mol Cell Biol. 1999 Oct;19(10):7245-54.

Insert

mitogen-activated protein kinase 8 interacting protein 2 (MAPK8IP2 )

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
pET28b-NmCas9-Flag

Plasmid

#109030

Purpose

Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Use

Tags

Flag Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE.puro/TO/Flag/HuR.wt

Plasmid

#110346

Purpose

Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG tag

Depositor

Sandra Martha Gomes Dias

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

gag

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCR-Flag-IKKalpha-KM

Plasmid

#15468

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase alpha-KM (Chuk Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Lysine at 44 to Alanine

Promoter

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
p3XFLAG-CMV 7.1_syn6

Plasmid

#50012

Purpose

Encodes syntaxin 6 with a N-terminal 3X FLAG tag

Depositor

David Hackstadt

Article

Moore et al Microbiology. 2011 Mar;157(Pt 3):830-8.

Insert

syntaxin 6 (Stx6 Rat)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-Rab7A Q86L

Plasmid

#28050

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Use

Tags

2xHA and 3xFlag

Expression

Mammalian

Mutation

Q86L

Promoter

Available since

June 22, 2011

Availability

Academic Institutions and Nonprofits only
FLAG-EglN2-pLenti6

Plasmid

#36950

Depositor

William Kaelin

Article

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

EglN2 (EGLN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wildtype

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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Flag-MCPIP1(1-555)

Plasmid

#90105

Purpose

Mammalian expression of Flag-MCPIP1(1-555)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk2a2

Plasmid

#15744

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2 (MAPK9 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
Flag-p50(1-435)

Plasmid

#44747

Depositor

Gourisankar Ghosh

Article

Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi

Insert

p50 (Nfkb1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

AA 1-435

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pFLAG-TNKS-2-M1054V

Plasmid

#34692

Depositor

Nai-Wen Chi

Article

Sbodio et al Biochem J. 2002 Feb 1;361(Pt 3):451-9.

Insert

TNKS2-M1054V (TNKS2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

M1054V

Promoter

CMV

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-EglN3-pLenti6

Plasmid

#36951

Depositor

William Kaelin

Article

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

EglN3 (EGLN3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wildtype

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1-WT 6KQ

Plasmid

#17562

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 6KQ (Foxo1 Mouse)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
MSCV-FlagmuEzh2deltaset-Hygro

Plasmid

#49403

Purpose

Expresses a Delta-set deletion mutant of Flag Tagged Ezh2. Hygromycin resistant.

Depositor

Tobias Neff

Insert

3prime end of Flag-mus-Ezh2deltaSET, eliminating Set domain

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Set domain deleted

Promoter

MSCV promotor

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+Flag-XTCF3-HA

Plasmid

#32998

Depositor

Sergei Sokol

Article

Hikasa et al Dev Cell. 2010 Oct 19;19(4):521-32.

Insert

XTCF3 (tcf3 Frog)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

sCMV IE94

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only