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Addgene

p3XFLAG-CMV 7.1_syn6
(Plasmid #50012)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 50012 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p3XFLAG-CMV 7.1
  • Backbone manufacturer
    Sigma Aldrich
  • Backbone size w/o insert (bp) 4717
  • Total vector size (bp) 5464
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    syntaxin 6
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    768
  • Entrez Gene
    Stx6
  • Promoter CMV
  • Tag / Fusion Protein
    • 3x FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R (5'-CCAGCTTGGTTCCCAATAGA-3')
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    eGFP-syntaxin 6 (J Biol Chem 275, 1261–1268) was used as a template to make the syntaxin 6 3XFLAG.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3XFLAG-CMV 7.1_syn6 was a gift from David Hackstadt (Addgene plasmid # 50012 ; http://n2t.net/addgene:50012 ; RRID:Addgene_50012)
  • For your References section:

    The trans-Golgi SNARE syntaxin 6 is recruited to the chlamydial inclusion membrane. Moore ER, Mead DJ, Dooley CA, Sager J, Hackstadt T. Microbiology. 2011 Mar;157(Pt 3):830-8. doi: 10.1099/mic.0.045856-0. Epub 2010 Nov 25. 10.1099/mic.0.045856-0 PubMed 21109560