p3XFLAG-CMV 7.1_syn6
(Plasmid
#50012)
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PurposeEncodes syntaxin 6 with a N-terminal 3X FLAG tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50012 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3XFLAG-CMV 7.1
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Backbone manufacturerSigma Aldrich
- Backbone size w/o insert (bp) 4717
- Total vector size (bp) 5464
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesyntaxin 6
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SpeciesR. norvegicus (rat)
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Insert Size (bp)768
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Entrez GeneStx6
- Promoter CMV
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Tag
/ Fusion Protein
- 3x FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (5'-CCAGCTTGGTTCCCAATAGA-3') (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byeGFP-syntaxin 6 (J Biol Chem 275, 1261–1268) was used as a template to make the syntaxin 6 3XFLAG.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3XFLAG-CMV 7.1_syn6 was a gift from David Hackstadt (Addgene plasmid # 50012 ; http://n2t.net/addgene:50012 ; RRID:Addgene_50012) -
For your References section:
The trans-Golgi SNARE syntaxin 6 is recruited to the chlamydial inclusion membrane. Moore ER, Mead DJ, Dooley CA, Sager J, Hackstadt T. Microbiology. 2011 Mar;157(Pt 3):830-8. doi: 10.1099/mic.0.045856-0. Epub 2010 Nov 25. 10.1099/mic.0.045856-0 PubMed 21109560