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Comp AlphaPS3
Plasmid#14071DepositorInsertDrosphila Comp Alpha PS3 Integrin (scb Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMT-HA-STX1
Plasmid#89310PurposeExpresses HA tagged syntaxin 1 in Drosophila cellsDepositorInsertSTX1 (Syx1A Fly)
UseTagsHA tagExpressionInsectMutationPromoterP-MT (Metallothionein)Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
K560GFP
Plasmid#129761PurposeExpresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assaysDepositorInsertKHC (Khc Fly)
UseTagsGFP and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-yki:V5-S111A, S168A, S250A
Plasmid#85620PurposeExpresses V5 tagged triple mutant yki in Drosophila.DepositorInsertyki-V5-S111A,S168,S250A
UseTagsV5ExpressionInsectMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT-TAK1-V5-His
Plasmid#63753PurposeExpresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorInsertTGF-β activated kinase 1 (Tak1 Fly)
UseTagsV5-HisExpressionInsectMutationPromoterpMTAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
KRigor980
Plasmid#129763PurposeExpresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfacesDepositorInsertKHC (Khc Fly)
UseTagsHis6ExpressionBacterialMutationR210APromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2-STOP-dsRed
Plasmid#60944PurposeDrosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.DepositorInsertdsRED
UseUnspecifiedTagsExpressionMutationPromoter3XP3Available sinceNov. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3xP3-EGFP.vas-int.NLS
Plasmid#60948PurposeDrosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.DepositorInsertvas-phiC31 integrase.NLS
UseRmceTagsExpressionMutationPromotervasaAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIHEU-MCS
Plasmid#58375PurposeUAS vector to express gene of interest in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
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act-AsCpf1
Plasmid#73917Purposeexpression plasmid of AsCpf1DepositorInserthAsCpf1
UseTagsNLS-3xHAExpressionInsectMutationPromoteract5CAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
K560_AviN_coBirA
Plasmid#129766PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSTEP
Plasmid#72334PurposeTissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)DepositorInsertRRS-TagRFPT-p10-[Golic+] RRS-EGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDsRed-TSTEP_v2
Plasmid#72336PurposeTissue-specifically swappable (C-terminal TagRFPT-to-EGFP) genome tagging plasmid with dsRed selection marker for injection mediated gene targeting (Drosophila melanogaster)DepositorInsertRRS-TagRFPT-p10-[3xP3dsRed]-RRS-EGFP
UseTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT EGFP SV40 pA
Plasmid#69911PurposeExpresses EGFP in DrosophilaDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
CvcE in pGEX4t
Plasmid#112977PurposeFor protein expression and purification of Drosophila CvcEDepositorInsertcycE (CycE Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CJ52 (cg1849 utr mut)
Plasmid#17780DepositorInsertcg1849 utr fragment mut
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationmutant versionPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
CJ53 (cg5166a/c utr)
Plasmid#17781DepositorInsertcg5166a/c utr fragment
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only
