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Plasmid#48177Purposecontains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame elementDepositorInsert5" UTR of 2KDR (MC2R Human)
UseLuciferaseTagsExpressionMutationA/G MUTATION IN ATG OF UORFPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac (GST-SNAP-Shp2)
Plasmid#177897PurposeBaculo expression of GST tag fused to SNAP tag and human Shp2DepositorInsertShp2 (PTPN11 Human)
UseTagsTEV-GST-PreScission cut site-SNAP tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a(ICOS)
Plasmid#177903PurposeBacterial expression of his10-tagged human ICOS Intracellular DomainDepositorInsertICOS (aa 162-199 only) (ICOS Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2(GST-SNAP-Shp2tSH2)
Plasmid#177907PurposeBaculo expression of GST tag fused to SNAPtag and human Shp2 tandem SH2 domainsDepositorInsertShp2 tandem SH2 domains (aa 6-218 only) (PTPN11 Human)
UseTagsGST-PreScission cut site-SNAP tagExpressionBacterialMutationPromotertacAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA
Plasmid#218528PurposesgRNA targeting human EIF2AK4/GCN2DepositorInsertEIF2AK4 (EIF2AK4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 asyn WT
Plasmid#107425PurposeMammalian expression of human alpha synucleinDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro-ARID1A
Plasmid#39478PurposeTetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vectorDepositorInsertARID1A (ARID1A Human)
UseLentiviralTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-hAXL
Plasmid#124797PurposeTet-inducible AXL expression in mammalian cellsDepositorInsertAXL (AXL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMVNeoDEST(705-1)-hAXL
Plasmid#124796PurposeExpresses AXL in mammalian cellsDepositorInsertAXL (AXL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
ADORA2A-Tango
Plasmid#66210PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertADORA2A (ADORA2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#75025PurposeCan be used to drive pan neuronal expression in zebrafish.DepositorInsertELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromoterelavl3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWAP-HGF
Plasmid#83503PurposeGeneration of transgenic mice overexpressing the HGF under the control of the WAP gene promoterDepositorInsertHGF (Hgf Mouse)
UseMouse TargetingTagsExpressionMutationalso contains beta-globin sequences from pUC198Promotermouse WAP (whey acidic protein)Available sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 asyn A30P
Plasmid#105729PurposeMammalian expression of mutant human alpha synucleinDepositorInsertalpha-synuclein A30P (SNCA Human)
UseTagsExpressionMammalianMutationA30PPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pP{ELAV-GeneSwitch}
Plasmid#83957PurposeExpresses conditional RU486-dependent GAL4 protein under control of the elav promotorDepositorInsertUseTagsExpressionInsectMutationPromoterelavAvailable sinceDec. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 asyn E46K
Plasmid#105730PurposeMammalian expression of mutant human alpha synucleinDepositorInsertalpha-synuclein E46K (SNCA Human)
UseTagsExpressionMammalianMutationE46KPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn E46K
Plasmid#105728PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein E46K (SNCA Human)
UseTagsExpressionBacterialMutationE46KPromoterT7Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH0006
Plasmid#135448PurposeHuman expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.DepositorInsertdCas9-BFP-KRAB
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNAI-GAL4-CREB
Plasmid#46769PurposeExpression of GAL4 fusion to wild type CREBDepositorUseTagsGAL4ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1a_FOXA1_P2A_Hygro_Barcode
Plasmid#120438PurposeBarcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertFOXA1 (FOXA1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-H2B-GCaMP6s
Plasmid#59530PurposeExpressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTransposable elementTagsExpressionMutationPromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only