Showing: 1721 - 1740 of 3020 results
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Plasmid#158153PurposeConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailabilityAcademic Institutions and Nonprofits only
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p32Lb
Plasmid#158154PurposeConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPlatTET-gRNA2
Plasmid#82559PurposeAll in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.DepositorInsertdCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MSP1594_2x_NLS
Plasmid#110625PurposeMammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)DepositorInsertsSt1Cas9 LMD-9
None
UseCRISPR and Synthetic BiologyTagsSV40 NLSExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pSH231-EFS-Cas9-BlastR
Plasmid#115146PurposeSafe harbor site 231 knock-in vector with Cas9-BlastR expression cassetteDepositorInsertCas9-T2A-BlastR
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP-SaCas9-HF (without sgRNA)
Plasmid#134470PurposeHuman expression vector for SaCas9-HF variant.DepositorInsertStaphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
UseCRISPRTagsSV40NLS-3xFLAGExpressionMammalianMutationR245A, N413A, N419A, R654APromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
Tet1-dCas9
Plasmid#136650PurposeTet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertTet1 (Tet1 Mouse)
UseCRISPRTags3XFLag-NLS-Tet1-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
DNMT3L-dCas9
Plasmid#126587PurposeDNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertDNMT3L (DNMT3L Human)
UseCRISPRTags3XFLag-NLS-DNMT3L-dCas9-NLSExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
dCas9-DNMT3L
Plasmid#126588PurposeDNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertDNMT3L (DNMT3L Human)
UseCRISPRTags3XFLag-NLS-dCas9-DNMT3L-NLSExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9
Plasmid#140237PurposeLentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3xFLAGExpressionMammalianMutationPromoterEF-1a coreAvailabilityAcademic Institutions and Nonprofits only -
pGG438
Plasmid#165486PurposeVector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAGDepositorInsertMammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
UseCRISPRTags3x FLAG and SV40 NLSExpressionMammalianMutationD1135V, S1136R, D1332K, and R1333G mutations in S…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pDGE687
Plasmid#167299PurposeVector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDGE792
Plasmid#167300PurposeVector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast
Plasmid#187320PurposedCas9 expression vector with cloning site for C terminal fusionsDepositorArticleTypeEmpty backboneUseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-hSt3Cas9 (pXK-1721)
Plasmid#208828PurposeCRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.DepositorTypeEmpty backboneUseCRISPR; Stcas9TagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRTagsExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailabilityAcademic Institutions and Nonprofits only -
pMJ824
Plasmid#39314DepositorInsertCas9
UseCRISPRTagsGST and His6ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pDD285
Plasmid#66826PurposemKate2^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmKate2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized mKate2ExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMD19T-slr0230-PL31-sgRNANT1-KmR
Plasmid#73221PurposeContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coliDepositorInsertPL31-sgRNA NT1
UseTagsExpressionBacterialMutationPromoterPL31AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-slr0230-PL22-sgRNANT1-KmR
Plasmid#73224PurposeContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coliDepositorInsertPL22-sgRNA NT1
UseTagsExpressionBacterialMutationPromoterPL22AvailabilityAcademic Institutions and Nonprofits only
Showing: 1721 - 1740 of 3020 results