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Plasmid#167887PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
mADAM17deltaMP in pQCXIP cFLAG
Plasmid#19144DepositorInsertADAM17 (Adam17 Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationdelta 189-378PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub-YPet-NES
Plasmid#138207PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_EBFP2
Plasmid#167881PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-shibire (ts1)
Plasmid#24362DepositorInsertShibire (ts1) (shi Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMM534
Plasmid#112530PurposepZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanRDepositorInsertluxCDABE
UseTagsExpressionBacterialMutationPromoterPL(HrtO)Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
mGFAP-Halo
Plasmid#169474Purposethe host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sitesDepositorInsertGFAP (Gfap Mouse)
UseTagsHaloTagExpressionMammalianMutationChanged R 236 to HPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
p35S::mCh-HA-P2A-lifeact-mCh
Plasmid#135234Purposeconstitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)DepositorInsertmCh-HA-P2A-lifeact-mCh
UseTagsHA, lifeact, and p2aExpressionPlantMutationPromoter35SAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
boP58-pBabe.puro(notag)
Plasmid#21885DepositorInsertP58 (DNAJC3 )
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-CeNLuc
Plasmid#135948PurposeExpresses the CeNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseTagsT2 mitochondrial localization sequenceExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
XE241 Rat frizzled-1-GFP-CS2P+
Plasmid#16817DepositorInsertfrizzled-1 (Fzd1 Rat)
UseXenopus expressionTagsGFPExpressionMutationPromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_EYFP
Plasmid#167882PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Venus-Sec61β
Plasmid#174467PurposeExpress Venus with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertVenus with endoplasmic reticulum-targeting sequence
UseTagsSec61 subunit betaExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL3
Plasmid#169108PurposeExpresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-Sec61β
Plasmid#174468PurposeExpress mCherry with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertVenus with endoplasmic reticulum-targeting sequence
UseTagsSec61 subunit betaExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLTR PKC beta II
Plasmid#8417DepositorInsertRat Protein Kinase C beta II (Prkcb Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-2xSV40_NLS-CeNLuc
Plasmid#135952PurposeExpresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseTags2xSV40-NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTH PKC alpha
Plasmid#8409DepositorInsertMouse Protein Kinase C alpha (Prkca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98N-pBabe-puro
Plasmid#19236DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 98 to AsparaginPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y112N-pBabe-puro
Plasmid#19238DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 112 to AsparaginPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only