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pSB-CMV-MCS-Puro dr_cPLA2-mKate2

Plasmid

#163844

Purpose

Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Tags

mKate2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS

Plasmid

#79373

Purpose

Recruits VPR to a compatible Cas9 protein for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

GCN4-sfGFP-VPR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHiUGE GS-gRNA (no Cre)

Plasmid

#200370

Purpose

Backbone plasmid for cloning HiUGE GS-gRNA (without Cre)

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Beclin1

Plasmid

#21150

Depositor

Junying Yuan

Article

Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

Insert

PcDNA3-Beclin1 (BECN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FXR1P

Plasmid

#198694

Purpose

Expression of human FXR1 protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FXR1P (FXR1 Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDisplay sHRPb-KDEL

Plasmid

#73150

Purpose

Small fragment of split HRP targeted for expression in the lumen of the endoplasmic reticulum (ER). CMV promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-KDEL

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Foxa2-IRES-GFP

Plasmid

#33008

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A2 (Foxa2 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pAct:dCas9

Plasmid

#78903

Purpose

Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

pActin (Drosophila)

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pHiUGE GS-gRNA (hSyn-Cre) backbone

Plasmid

#200368

Purpose

Backbone plasmid for cloning HiUGE GS-gRNA (with hSyn-Cre)

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE HA-epitope donor ORF0

Plasmid

#200371

Purpose

Tagging endogenously expressed proteins with HA epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

HA epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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eTC GFP beta-actin full length

Plasmid

#27123

Depositor

Robert Singer

Article

Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

Insert

beta-actin (ACTB Human)

Use

Tags

EGFP and TC tag

Expression

Mammalian

Mutation

E237A

Promoter

Available since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pKH159_FLAG-APEX2-YIPF4 WT

Plasmid

#214978

Purpose

Flag-APEX2 tagged YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF4 (YIPF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHiUGE HA-epitope donor ORF2

Plasmid

#200373

Purpose

Tagging endogenously expressed proteins with HA epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

HA epitope

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET14-GFP30_Encap

Plasmid

#86406

Purpose

Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 30aa CLP tag
Encapsulin

Use

Tags

6x Histidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-FIP3

Plasmid

#74753

Purpose

Expressed sfGFP-tagged FIP3 in E. coli for protein production

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Rab11 family interacting protein 3 (RAB11FIP3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-c-Myc-IP

Plasmid

#15921

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (Myc Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 C506S

Plasmid

#78584

Purpose

Expression flag-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 C506S (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

mutate 506 cysteine residue to serine

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pYPQ210

Plasmid

#109329

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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