-
Plasmid#49005PurposeMammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2DepositorInsertets variant gene 2 (etsrp Zebrafish)
UseInvitro transcription, riboprobe templateTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
(K) tNOS (S)_T3 (SBE120)
Plasmid#195010Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)DepositorInsert(K) tNOS (S)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(H) tNOS (I) _T2 (SBE119)
Plasmid#195006Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)DepositorInsert(H) tNOS (I)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(E) tNOS (F) _T1 (SBE118)
Plasmid#194997Purposeterminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)DepositorInsert(E) tNOS (F)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
(I) pXYL (J) P3 (SBE108)
Plasmid#194991Purposepromoter of the gene xylose reductase from R. toruloides for a position P3DepositorInsert(I) pXYL (J)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FFiBlast MSCV Puro
Plasmid#68464PurposeRetroviral plasmid for establishment of blasticidin resistance Cre-reporter cell lineDepositorInsertBlasticidin Resistance
UseCre/Lox and RetroviralTagsExpressionMammalianMutationInvertedPromoterLTRAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP120-TD-HilA
Plasmid#153330PurposeTriggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinoseDepositorInsertSptP120-HA4-7c12 Tandem Monobody
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK2
Plasmid#118092PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 2 (SPHK2 Human)
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGoldyTALEN-TUBA1B-TAL-2
Plasmid#62854PurposeTALEN mediated gene editing at human TUBA1B locusDepositorInsertTALEN array
UseTALENTagsExpressionMammalianMutationPromoterminiCAGGAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGoldyTALEN-TUBA1B-TAL-1
Plasmid#62853PurposeTALEN mediated gene editing at human TUBA1B locusDepositorInsertTALEN array
UseTALENTagsExpressionMammalianMutationPromoterminiCAGGAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS
Plasmid#107317PurposeExpresses SaCas9 in mammalian cellsDepositorInsertSaCas9
UseCRISPRTags3xHA and 2xNLS and NLSExpressionMammalianMutationPromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
LC501: pMVP (L3-L2) mCherry + polyA
Plasmid#121765PurposepMVP L3-L2 entry plasmid, contains mCherry + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest.DepositorInsertmCherry + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
JN607: pMVP/SB-DEST
Plasmid#121860PurposepMVP destination vector, empty Sleeping Beauty transposon vector backboneDepositorTypeEmpty backboneUseSleeping beauty transposon, pmvp gateway destinat…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HB901: pMVP (L3-L2) polyA
Plasmid#121746PurposepMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertPolyadenylation signal (polyA)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IF701: pMVP (L5-L4) APEX2
Plasmid#121725PurposepMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.DepositorInsertAPEX2 (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAflaB
Plasmid#149588Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP167-3G124-HilA
Plasmid#153322PurposeTriggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP167-3G124 DARPin
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
KP201: pMVP (L5-L4) eGFP-P2A
Plasmid#121708PurposepMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.DepositorInserteGFP-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMEH305 HA NLS SpCas9 3x high affinity MS2 loops
Plasmid#168216PurposeMammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tagDepositorInsertSV40 NLS SpCas9
UseLentiviralTagsHA, SV40 NLS, and nucleoplasmin NLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
KP301: pMVP (L5-L4) mCherry-P2A
Plasmid#121710PurposepMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.DepositorInsertmCherry-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only