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Plasmid#109790PurposeProtein expression and purification of VASP_WH1DepositorInsertVASP_WH1 (VASP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PLEK2_DEP
Plasmid#109798PurposeProtein expression and purification of PLEK2_DEPDepositorInsertPLEK2_DEP (PLEK2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCool-human TSC1 (939-992)
Plasmid#132618Purposeexpresses human TSC1 (939-992) in E. coliDepositorInsertTSC1 (939-992)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ATG-3xHA-S11
Plasmid#108238PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (3xHA-S11 as control) in plantsDepositorInsertATG-3xHA-S11
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-3nmt1-mEOS3.2
Plasmid#105166PurposeYeast gene targetingDepositorInsertkanMX6-3nmt1-mEOS3.2
UseTags3nmt1-mEOS3.2ExpressionBacterial and YeastMutationPromoter3nmt1Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
gamma-tubulin (334-451)
Plasmid#104436Purposeexpressing a fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLSDepositorInsertgamma-tubulin fragment 334 to 451 (TUBG1 Human)
UseTagsNoneExpressionMammalianMutationdeleted a.a. 1 to 333PromoterAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMflT-o2
Plasmid#101310PurposeContains: oriC2 fragment of M. florum L1 replication origin (dnaA/dnaN intergenic region), colE1 replication origin, recoded tetM resistance gene.DepositorInsertoriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM5080
Plasmid#85106PurposePtrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expresDepositorInsertYFP-KaiC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM5081
Plasmid#85107PurposePtrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.DepositorInsertYFP-KaiC
UseTagsYFPExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRFP shRNA-resistant TrkB F616A -S478A
Plasmid#72718PurposeMammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant and lacking TIAM1-binding siteDepositorInsertTrkB
UseTagsRFPExpressionMammalianMutationshRNA-resistant TrkB (T300C, T301A, C302G, C306A,…PromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR SS287AA
Plasmid#72703PurposeLentiviral expression of GR Phospho-deficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationSS287AAPromoterubiquitinAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)Q30D
Plasmid#60346PurposeQ30D mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationQ30D; contains amino acids 7-192 of Msi1PromoterT7Available sinceDec. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R53E
Plasmid#60352PurposeR53E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR53E; contains amino acids 7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G64A
Plasmid#60351PurposeG64A mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG64A; contains amino acids 7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)H83L
Plasmid#60350PurposeH83L mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationH83L; contains amino acids 7-192 of Msi1PromoterT7Available sinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G35D
Plasmid#60347PurposeG35D mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG35D; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)G35E
Plasmid#60348PurposeG35E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationG35E; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)H83F
Plasmid#60349PurposeH83F mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationH83F; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTH443-SUP45-G180A
Plasmid#29386DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationG180A mutant gene including genomic sequence 1432…PromoterAvailable sinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH398-SUP45-G54D
Plasmid#29376DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationG54D mutant gene including genomic sequence 1432 …PromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only