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Plasmid#65157PurposeEncodes sgRNA Dropout as a Type 234 part to be used in the Dueber YTK systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only
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Construct 9 - U6p_30nt_stgRNA
Plasmid#81248PurposeExpresses 30nt stgRNADepositorInsertsgRNA
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Construct 10 - U6p_40nt_stgRNA
Plasmid#81249PurposeExpresses 40nt stgRNADepositorInsertsgRNA
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFD5
Plasmid#73914Purposemultiplex gRNA plasmidDepositorInsertdU6-3:tRNA:gRNA:tRNA:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP2844
Plasmid#160675PurposeMobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDonor_sU6
Plasmid#69351PurposesgRNA scaffold and synthetic U6 promoterDepositorInsertsU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRTagsExpressionMutationPromotersynthetic U6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDonor_mU6
Plasmid#69350PurposesgRNA scaffold and mouse U6 promoterDepositorInsertmU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRTagsExpressionMutationPromotermU6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDonor_hU6
Plasmid#69312PurposesgRNA scaffold and human U6 promoterDepositorInserthU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRTagsExpressionMutationPromoterhuman U6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP2846
Plasmid#160676PurposeMobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMLS134
Plasmid#73714PurposeSapTrap destination vector for building sgRNA expression vectors.DepositorInsertpU6::sgRNA
UseCRISPRTagsExpressionWormMutationSapI insertion sitePromoterC. elegans U6 snRNA pol III promoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pV1090
Plasmid#111427PurposegRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)DepositorInsertsgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
ttHsCas12a
Plasmid#182385PurposeGives high editing efficiency in barley.DepositorInsertttHsCas12a
UseCRISPR; Coding sequence onlyTagsExpressionMutationD156RPromoterNoneAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP2849
Plasmid#160677PurposeMobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-TelgRNA_PuroR
Plasmid#84782PurposeExpresses telomere-specific sgRNA.DepositorInserttelomere-specific sgRNA
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMLS256
Plasmid#73715PurposeSapTrap destination vector for building combined sgRNA expression + repair template vectorsDepositorInsertpU6::sgRNA
UseCRISPRTagsExpressionWormMutationSapI insertion sitePromoterC. elegans U6 snRNA pol III promoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJW1259
Plasmid#61251PurposeCas9 plasmid with sgRNA deleted; for use in C. elegansDepositorInsertDeletion
UseCRISPRTagsExpressionWormMutationDeleted sgRNA(F+E) sequence from pJW1219PromoterAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTK372
Plasmid#114687PurposesgRNA targeting NuMA's C-terminal locusDepositorInsertNuMA sgRNA
UseTagsExpressionMutationPromoterAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT055g
Plasmid#96864PurposesgRNA-HEK3DepositorInsertsgRNA-HEK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMP2782
Plasmid#160668PurposeMobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ2048.2
Plasmid#140687Purpose3-color stripe (different sgRNAs)DepositorInsert3-color stripe (different sgRNAs)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only