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FUS_LC_charge_RtoK

Plasmid

#139127

Purpose

express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Add K and D to FUS LC so it has the same charge p…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_charge

Plasmid

#139126

Purpose

express FUS LC with an hnRNPA2 LC-like charge patterning

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Use

Tags

Expression

Bacterial

Mutation

Add R, K, D to make FUS LC have similar charge pa…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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ORAI1-YFP V107M-L273D-L276D

Plasmid

#114183

Purpose

ORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.

Depositor

Nicolas Demaurex

Article

Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

Insert

ORAI1-YFP V107M-L273D-L276D (ORAI1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

V107M/L273D/L276D

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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ORAI1-YFP T184M-L273D-L276D

Plasmid

#114184

Purpose

ORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.

Depositor

Nicolas Demaurex

Article

Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

Insert

ORAI1-YFP T184M-L273D-L276D (ORAI1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

T184M/L273D/L276D

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_WT_S285C

Plasmid

#98665

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

S285C

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_D290V_S285C

Plasmid

#98666

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C and D290V

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

S285C D290V

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_WT_S329C

Plasmid

#98667

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S329C

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

S329C

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_D290V_S329C

Plasmid

#98668

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V and S329C

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

D290V S329C

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_D290V

Plasmid

#98658

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

D290V

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_D220V

Plasmid

#98659

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D220V

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

D220V

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPA2_LC_D230V

Plasmid

#98660

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D230V

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

D230V

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_P298L

Plasmid

#104465

Purpose

express His tagged P298L hnRNPA2 LC

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

P298L

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_LC_R191K_R254K

Plasmid

#104466

Purpose

express MBP hnRNPA2 LC with 2 R to K mutations

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

His-MBP

Expression

Bacterial

Mutation

R191K R254K

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_P298L_S329C

Plasmid

#104470

Purpose

express His tagged P298L S329C hnRNPA2 LC

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

P298L S329C

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-PSAT

Plasmid

#74115

Purpose

Expression of PSAT

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

pET28a-PSAT (PSAT1 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-PHGDH

Plasmid

#74113

Purpose

Expression of N-terminal His-tagged

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

PHGDH (PHGDH Human)

Use

Tags

His-tagged

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAR-IF reporter

Plasmid

#16628

Depositor

Bert Vogelstein

Article

Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

Insert

CAR-IF

Use

Tags

beta-galactosidase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pCAR-OF reporter

Plasmid

#16627

Depositor

Bert Vogelstein

Article

Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

Insert

CAR-OF

Use

Tags

beta-galactosidase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pCYP55

Plasmid

#212888

Purpose

Expresses the Salinispora arenicola LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Use

Tags

Expression

Bacterial

Mutation

Promoter

PcymRC

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pJ4M/TDP-43

Plasmid

#104480

Purpose

bacterial expression of TDP-43

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Use

Tags

MBP-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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