Addgene

We narrowed to 4,790 results for: puc

Showing: 1621 - 1640 of 4790 results

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CJ47 (U1 snRNA (U1;21d) 3G mutant)

Plasmid

#17776

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

U1 snRNA (U1;21d) (snRNA:U1:21D Fly)

Use

Tags

Expression

Mutation

3G mutant

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

Plasmid

#46168

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

codon optimized Cas9_SV40 NLS with intron

Use

CRISPR

Tags

Expression

Mutation

intron inserted at position 3113-3163, and SV40 N…

Promoter

eef-1A.1 (eft-3)

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pNC1124

Plasmid

#41560

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-GAL1-UBIYdkGFP*-SpHis5-Tim9

Use

Tags

Expression

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Promoter

Available since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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pNC1125

Plasmid

#41561

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-GAL1-UBIMdkGFP*-SpHis5-Tim9

Use

Tags

Expression

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Promoter

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pNC1136

Plasmid

#41562

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9

Use

Tags

Expression

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Promoter

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pNC1137

Plasmid

#41563

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-FUS1-UBIMdkGFP*-SpHis5-Tim9

Use

Tags

Expression

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Promoter

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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23SrRNA.RAV12(wt).2508-2580.G2530AAAC

Plasmid

#26190

Depositor

David McKay

Article

Hardin et al J Mol Biol. 2010 Jul 29. ():.

Insert

23S ribosomal RNA fragment (modified)

Use

Tags

Expression

Bacterial

Mutation

fragment 2508-2580 of E. coli rRNA, with nucleoti…

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pPLP1.3

Plasmid

#22615

Depositor

Lynn Hudson

Article

Puckett et al J Neurosci Res. 1987 . 18(4):511-8.

Insert

Proteolipid Protein

Use

E. coli phagemid vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pPLP1.5

Plasmid

#22614

Depositor

Lynn Hudson

Article

Puckett et al J Neurosci Res. 1987 . 18(4):511-8.

Insert

human Proteolipid Protein cDNA (PLP1 Human)

Use

E. coli phagemid vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pMT1100

Plasmid

#8618

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

ribonuclease Sa3, barstar

Use

Tags

phoA signal sequence to RNase Sa3

Expression

Bacterial

Mutation

In addition to the substitution of the barnase ge…

Promoter

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
23SrRNA.RAV12(wt).2508-2580.GAAAC.duplexH90

Plasmid

#26192

Depositor

David McKay

Article

Hardin et al J Mol Biol. 2010 Jul 29. ():.

Insert

23S ribosomal RNA fragment (modified) with duplex H90

Use

Tags

Expression

Bacterial

Mutation

fragment 2508-2580 of E. coli rRNA, with nucleoti…

Promoter

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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23SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U

Plasmid

#26191

Depositor

David McKay

Article

Hardin et al J Mol Biol. 2010 Jul 29. ():.

Insert

23S ribosomal RNA fragment (modified) with G2553U mutation

Use

Tags

Expression

Bacterial

Mutation

fragment 2508-2580 of E. coli rRNA, with nucleoti…

Promoter

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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35S:5mARF17 pLBR19

Plasmid

#12073

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

5mARF17 (ARF17 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

miR160-resistant version of ARF17 has five silent…

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pChimera

Plasmid

#61476

Purpose

Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

U6-26:sgRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

U6-26

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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Drp1(A395D)-StrepII

Plasmid

#174430

Purpose

Expresses human Drp1 Isoform 3 with A395D mutation in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

StrepII

Expression

Bacterial

Mutation

A395D

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Drp1-StrepII

Plasmid

#174428

Purpose

Expresses human Drp1 Isoform 3 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSCW01

Plasmid

#72300

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSCW02

Plasmid

#72301

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ10873

Plasmid

#183961

Purpose

U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Inserts

HyperdCas12a
poly-crRNA array targeting Sox2-Klf4-Oct4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG and U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAS

Plasmid

#60847

Purpose

Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

Depositor

Jamie Cate

Article

Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

Inserts

S. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA

Use

CRISPR and Synthetic Biology

Tags

NLS/His8 and TTT 3' extension prior to sgRNA

Expression

Bacterial and Yeast

Mutation

L4 is UUCG tetraloop and guide targets LYP1 (CATA…

Promoter

Available since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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