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We narrowed to 108,114 results for: plasmid

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Showing: 1621 - 1640 of 108114 results

  1. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Mutation
    Silent change from C to A at bp 834 of insert
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45945-plasmid-map-sequence-id-116251

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  4. pAAV-Ef1a-DIO EYFP (AAV Retrograde)

    Viral prep
    #27056-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, Cre-dependent EYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Tags
    EYFP (Cre-dependent)
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only

  5. pAAV-hsyn-flex-Voltron-ST (AAV1)

    Viral prep
    #119036-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hsyn-flex-Voltron-ST (#119036). In addition to the viral particles, you will also receive purified pAAV-hsyn-flex-Voltron-ST plasmid DNA. Synapsin-driven, cre-dependent expression of genetically encoded fluorescent voltage indicator Voltron1 with a soma-targeting sequence. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Eric Schreiter
    Publication
    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641
    Promoter
    Syn
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only

  6. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV PHP.eB)

    Viral prep
    #104492-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. pENN.AAV.hSyn.TurboRFP.WPRE.RBG (AAV1)

    Viral prep
    #105552-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.hSyn.TurboRFP.WPRE.RBG (#105552). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.TurboRFP.WPRE.RBG plasmid DNA. hSynapsin-driven TurboRFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    TurboRFP
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. lentiGuide-Hygro-mTagBFP2

    Plasmid
    #99374
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99374-plasmid-map-sequence-id-430620

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  10. lentiGuide-Hygro-mTagBFP2

    Plasmid
    #99374
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-mTagBFP2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99374-plasmid-map-sequence-id-430620

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  15. pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV9)

    Viral prep
    #83897-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Publication
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    hDlx
    Tags
    dTomato
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV PHP.eB)

    Viral prep
    #100854-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only

  17. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Publication
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73796-plasmid-map-sequence-id-237125

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  18. pAAV-GFAP-hM4D(Gi)-mCherry

    Plasmid
    #50479
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter
    Depositor
    Bryan Roth
    Has service
    AAV5
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50479-plasmid-map-sequence-id-166303

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  20. pCBC-DT1T2

    Plasmid
    #50590
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, U626t plus, U629p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50590-plasmid-map-sequence-id-210724

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Showing: 1621 - 1640 of 108114 results