We narrowed to 58,181 results for: plasmid
-
Plasmid#60224PurposeExpresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.DepositorInsertsUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HA and Rluc-P2AExpressionMammalianPromoterEFS, No promoter, and U6Available SinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only
-
pDAC439
Plasmid#195237PurposeMammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)DepositorInsertFLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
ExpressionMammalianAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_LaG16_2_synNotch_Gal4VP64
Plasmid#85421PurposeLentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic NotchDepositorInsertLaG16_2 GFP nanobody synNotch Gal4VP64
UseLentiviralPromoterPGKAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pComb3XTT
Plasmid#63891PurposeBackbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)DepositorInsertTT Fab
UsePhage displayTags6xHis, HA tag, and geneIIIExpressionBacterialPromoterlacZAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-SOD2-2A-Catalase-WPRE
Plasmid#67635PurposeAAV vector expressing both SOD2 and mitochondrial targeted CatalaseDepositorUseAAVMutationDeleted the Peroxisome Targeting Signal from Cata…PromoterCMVAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-Nanog-IP
Plasmid#13838DepositorAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCherry-Klf4
Plasmid#23243DepositorInsertmCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)
UseLentiviralAvailable SinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLDLR-Luc (aka: pES7)
Plasmid#14940DepositorAvailable SinceMay 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
HIP-flash (HOP-flash mutant)
Plasmid#83466PurposeNegative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti U6-sgRNA/EF1a-mCherry
Plasmid#114199PurposeLentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoterDepositorInsertsgRNA scaffold
UseLentiviralTagsmCherryExpressionMammalianPromoterU6Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-sfGFP
Plasmid#85482PurposeArabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
Tags6x HisExpressionBacterialPromoterArabinoseAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCB320
Plasmid#89359Purpose3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sitesDepositorInsertpuromycin resistance, mCherry
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDW1775
Plasmid#13456PurposePlant ZFN expression vector.DepositorTypeEmpty backboneTagsAcV5ExpressionPlantAvailable SinceDec. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
anti-FLAG M2 heavy chain
Plasmid#175359PurposeMammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)DepositorInsertanti-FLAG M2 heavy chain
TagsHis8 tag and N-terminal secretion signal peptide …ExpressionMammalianMutationL51I (see paper)PromoterCMVAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Flag-cMyc T58A
Plasmid#20076DepositorAvailable SinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCBFRE-luc
Plasmid#26897DepositorInsertCBFRE
UseLuciferaseTagsluciferaseExpressionMammalianAvailable SinceDec. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-CD3z
Plasmid#135993PurposeModular CD3z alone CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralExpressionMammalianPromoterEF1a-shortAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40
Plasmid#101060PurposeAAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas ServiceAAV1InsertCaMPARI2
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mTH-GFP
Plasmid#99128PurposeAn AAV genome that expresses GFP from the mouse TH promoterDepositorInsertGFP
UseAAVExpressionMammalianPromotermTHAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only