We narrowed to 105,129 results for: plasmid
-
Viral prep#27056-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, Cre-dependent EYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorTagsEYFP (Cre-dependent)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)
Viral prep#100798-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins
Pooled library#101929PurposeBassik lab Human CRISPR-Cas9 Deletion Library - Membrane proteins. Coexpresses mCherry.DepositorExpressionMammalianUseCRISPRAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
Plasmid#73796PurposepRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNADepositorInsertsdCas9-Mxi1
Tet Repressor
Structural gRNA for S pyogenes
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1, pRPR1(TetO), and pTef1Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-GFAP-hM4D(Gi)-mCherry
Plasmid#50479PurposeGi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoterDepositorHas serviceAAV5InserthM4D(Gi)-mCherry
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV PHP.eB)
Viral prep#100854-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV5 trial size)
Viral prep#37825-AAV5.TPurposeReady-to-use AAV5 trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-flex-Voltron-ST (AAV1)
Viral prep#119036-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hsyn-flex-Voltron-ST (#119036). In addition to the viral particles, you will also receive purified pAAV-hsyn-flex-Voltron-ST plasmid DNA. Synapsin-driven, cre-dependent expression of genetically encoded fluorescent voltage indicator Voltron1 with a soma-targeting sequence. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-GCaMP6s
Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tol2-elavl3-GCaMP6s
Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pdCas9
Plasmid#46569PurposeExpresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.DepositorPublicationInsertstracrRNA
dcas9
CRISPR array
UseCRISPRExpressionBacterialMutationD10A, H840AAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV9)
Viral prep#83897-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomatoAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PE8c
Plasmid#257699Purposein vitro transcription plasmid for PE8cDepositorInsertPE8c prime editor
UseTemplate for in vitro transcriptionAvailable sinceJune 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_zeo backbone
Plasmid#61427Purpose3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ104 - Pmyo-3::mCherry::unc-54
Plasmid#19328DepositorPublicationInsertPmyo-3::mCherry
TagsmCherryExpressionWormAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ90 - Pmyo-2::mCherry::unc-54utr
Plasmid#19327DepositorPublicationInsertPmyo-2::mCherry
ExpressionWormAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCM-AAVS1-TO-hNGN2
Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TRE-EGFP-EF1a-rtTA
Plasmid#104454PurposePiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotorDepositorInsertsenhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3
UsePiggybacExpressionMammalianAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCBC-DT1T2
Plasmid#50590PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, U626t plus, U629p, T2
UseCRISPR; Pcr templateExpressionPlantAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV Puro
Plasmid#68469PurposeRetroviral plasmid with multiple cloning site and puromycin resistanceDepositorInsertPuromycin Resistance
UseRetroviralExpressionMammalianPromoterpGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by