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We narrowed to 59,482 results for: plasmid

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Showing: 1621 - 1640 of 59482 results

  1. NL4-3 mCherry Luciferase

    Plasmid
    #44965
    Purpose
    HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.
    Depositor
    Warner Greene
    Publication
    Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371
    Insert
    mCherry, Luciferase, IRES
    Use
    Lentiviral
    Tags
    Luciferase, IRES and mCherry with D212G
    Mutation
    S34C mutation in Nef
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    44965-plasmid-map-sequence-id-352462

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  3. pC0040-LwaCas13a crRNA backbone

    Plasmid
    #103851
    Purpose
    Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6-LwaCas13aDR-BbsI-BbsI-polyT
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103851-plasmid-map-sequence-id-199180

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  9. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

    Plasmid
    #84563
    Purpose
    Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP
    Depositor
    Robert Singer
    Publication
    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d
    Insert
    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
    Use
    Lentiviral
    Tags
    myc tag (9x)
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84563-plasmid-map-sequence-id-176790

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  15. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71667-plasmid-map-sequence-id-243369

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  19. pLV-TetO-hNGN2-Puro

    Plasmid
    #79049
    Purpose
    Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15
    Insert
    hNGN2-P2A-PuroR (NEUROG2 Human)
    Use
    Lentiviral
    Promoter
    TetO
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79049-plasmid-map-sequence-id-429069

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Showing: 1621 - 1640 of 59482 results