Addgene

We narrowed to 5,233 results for: pCas

Showing: 1621 - 1640 of 5233 results

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pCAGGS-Flag-hsDicer (D1320A)

Plasmid

#41586

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

D1320A

Promoter

CMV

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

Plasmid

#107305

Purpose

Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells

Depositor

Scot Wolfe

Insert

SpCas9

Use

CRISPR

Tags

2x NLS and FKBP

Expression

Mammalian

Mutation

R1335K and fused to FKBP

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-pac-T2A-iCre

Plasmid

#113849

Purpose

Puromycin resistance gene and iCre recombinase

Depositor

Mario Capecchi

Insert

pac-T2A-iCre

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCA-T-intron(Neo)-G(LNL)

Plasmid

#36908

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides before nucleotide 275 and ins…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCasper-pNos-PCP-eGFP

Plasmid

#180274

Purpose

to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

Depositor

Hernan Garcia

Article

Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

Insert

PCP-eGFP

Use

Tags

eGFP

Expression

Insect

Mutation

Promoter

nanos

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-eSpCas9-D10A

Plasmid

#80449

Purpose

enhanced SpCas9 mammalian expression vector

Depositor

Ervin Welker

Insert

ehSpCas9_D10A

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEN396-pCAGGS-TIR1-V5-2A-HygroR

Plasmid

#195102

Purpose

Vector to introduce osTir1 in cells. Hygromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

TIR1

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-LplA(AILR,36)-NRX3b

Plasmid

#43919

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

AILR-LplA36-NRX3b (lplA Human, Synthetic)

Use

Tags

2XHA

Expression

Mammalian

Mutation

Promoter

Chicken beta actin

Available since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pSpCas9-Puro HLAG-2B-sgRNA

Plasmid

#182552

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2B-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pScalps eSpCas9_Zsgreen gRNA scaffold

Plasmid

#188449

Purpose

Expression of eSpCas9 tagged with Zsgreen

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF1-alpha

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCASP-HilA empty vector

Plasmid

#153333

Purpose

Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Type

Empty backbone

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

Plasmid

#148855

Purpose

Mammalian Expression of HsAGO3-sgRNA

Depositor

Elisa Izaurralde

Insert

HsAGO3-sgRNA (AGO3 S.pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GBP-dCas9-mRFP

Plasmid

#102933

Purpose

Expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

Depositor

Sebastian Bultmann

Article

Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

Insert

dCas9

Use

CRISPR

Tags

GBP (GFP binding protein) and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only
pMS24: pCascade(PmcCAST)_entry

Plasmid

#168157

Purpose

Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP167-3G124-HilA

Plasmid

#153322

Purpose

Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP167-3G124 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-eSpCas9-H840A

Plasmid

#80454

Purpose

enhanced SpCas9 mammalian expression vector

Depositor

Ervin Welker

Insert

ehSpCas9_H840A

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

Plasmid

#128174

Purpose

Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-iCreERT2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PB1-MontiRed-LOV2

Plasmid

#178872

Purpose

Mammalian expression of PB1-MontiRed-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-MontiRed-LOV2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Trex2-bpA-EF1BFP

Plasmid

#111144

Purpose

Expression of mouse Trex2 and BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

mouse Trex2 coding region (Trex2 Mouse)
BFP (Rnf112 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG and EF1

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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