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Plasmid#69245PurposeTet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a random RRNA spacer locus in T. brucei.DepositorTypeEmpty backboneUseExpression in trypanosoma bruceiTagsExpressionMutationPromoterRRNA promoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only
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pFREE
Plasmid#92050PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionBacterialMutationPromoterpRhamBAD and ptetAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA (V5 and HA)
Plasmid#222485PurposeCo-expression of two genes in yeast with V5 and HA tags and URA3 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-TRP (V5 and HA)
Plasmid#222488PurposeCo-expression of two genes in yeast with V5 and HA tags and TRP1 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-HIS (V5 and HA)
Plasmid#222487PurposeCo-expression of two genes in yeast with V5 and HA tags and HIS3 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU (V5 and HA)
Plasmid#222486PurposeCo-expression of two genes in yeast with V5 and HA tags and LEU2 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Zeo
Plasmid#92053PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Amp
Plasmid#92051PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJB06
Plasmid#190480PurposeBase Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsCas9
XylR
UseE. coli / b. subtilis - c. difficile shuttle vect…TagsExpressionMutationPromoterxylR-driven promoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB07
Plasmid#190481PurposeTargeting plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsUpstream & Downstream pyrE deletion region
gRNA
UseE. coli / b. subtilis - c. difficile shuttle vect…TagsExpressionMutationPromoterAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Spike-d18
Plasmid#164583PurposeA mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.DepositorInsertSARS-CoV-2 Spike (S SARS-CoV-2)
UseTagsExpressionMammalianMutationDeleted amino acids 1256-1273PromoterEF1aAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pFG036 + pFG051
Plasmid#137997PurposeTransposon mutagenesis plasmid and the cI repressor expression plasmidDepositorInsertTn5 tnp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA
Plasmid#194247PurposeExpresses 3 miRNAs targeting human alpha-SynucleinDepositorInsertmiRaSynuclein(human) (SNCA Human)
UseAAV and RNAiTagsExpressionMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCF1135
Plasmid#222106PurposeE. coli - Veillonella shuttle plasmid with single selectable markerDepositorTypeEmpty backboneUseE. coli-veillonella shuttle plasmidTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFREE_Cm
Plasmid#92052PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-TdTomato-RAB11
Plasmid#203732PurposeAAV vector plasmid expressing human RAB11 fused to TdTomato under the human synapsin (SYN) promoterDepositorInsertRAB11 (RAB11A Human)
UseAAVTagsTdTomatoExpressionMammalianMutationPromoterHuman synapsin promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-eGFP-RAB11
Plasmid#203731PurposeAAV vector plasmid expressing human RAB11 fused to eGFP under the human synapsin (SYN) promoterDepositorInsertRAB11 (RAB11A Human)
UseAAVTagseGFPExpressionMammalianMutationPromoterHuman synapsin promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM3558
Plasmid#40252DepositorInsertGm cassette (BssHII+SphI)
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCUX1.1.0-gDNA
Plasmid#112434PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1DepositorInsertCUX1 (CUX1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only