Addgene

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Showing: 1581 - 1600 of 9057 results

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pME-QF2w-SV40pA

Plasmid

#155117

Purpose

Middle entry vector containing QF2w

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QF2w

Use

Gateway middle entry vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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A104T DJ-1 pET15b

Plasmid

#60680

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

A104T DJ-1 pET15b (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A104T

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
H2B-MiniR-mEm

Plasmid

#181990

Purpose

MiniR genetically encoded tag on H2B-mEmerald to be used with MiniE labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

H2B-MiniR-mEm

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRC05

Plasmid

#114684

Purpose

FKBP-TEV-C

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

FKBP-TEV-C

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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MXS_minPGK

Plasmid

#62415

Purpose

MXS_chaining vector with minPGK

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Minimal PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G12A

Plasmid

#83142

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12A mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G12A

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-NHERF1

Plasmid

#31650

Depositor

Edward Weinman

Article

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Use

Tags

His & S tag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2G09

Plasmid

#70923

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

E(z) (E(z) Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
pJZ002

Plasmid

#120231

Purpose

CRISPR-cas9 targeting in E. coli with ampicillin resistance

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRC04

Plasmid

#114683

Purpose

FRB-TEV-N

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

FRB-TEV-N

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Tac Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTSA-38

Plasmid

#17328

Depositor

Takeshi Sano

Article

Sano et al Proc Natl Acad Sci U S A. 1995 Apr 11. 9

Insert

streptavidin

Use

Tags

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Promoter

Available since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only
phpRL-BCL2-FL-pA

Plasmid

#42595

Depositor

Richard Lloyd

Article

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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MXS_CAG

Plasmid

#62420

Purpose

MXS_chaining vector with CAG

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CAG Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_bGHpA

Plasmid

#62425

Purpose

MXS_chaining vector with bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Bovine growth hormone poly A

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2G08

Plasmid

#70915

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Parg (Parg Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
AtFLS2pro::GUS

Plasmid

#87167

Purpose

expression pattern of AtFLS2

Depositor

Silke Robatzek

Article

Beck et al J Exp Bot. 2014 Dec;65(22):6487-98. doi:

Insert

AT5G46330 promoter

Use

Tags

GUS

Expression

Mutation

Promoter

AtFLS2

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR/pTER shEGFP #2 (728-2)

Plasmid

#17471

Purpose

Gateway entry vector for EGFPshRNA#2 under H1/TO promoter

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #2

Use

RNAi; Entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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p415 GFP-Glo3

Plasmid

#112658

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

sfGFP-Glo3

Use

Tags

sfGFP

Expression

Yeast

Mutation

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b C185S

Plasmid

#83139

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), C185S mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

C185S

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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