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Plasmid Kit#1000000039PurposeInsert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systemsDepositorAvailable sinceApril 2, 2014AvailabilityAcademic Institutions and Nonprofits only
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MoClo Yeast Secretion and Display (YSD) Toolkit
Plasmid Kit#1000000240PurposeThe YSD Toolkit is a collection of 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK) to optimize secretion and cell surface display of proteins of interest in yeast.DepositorAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ubiquiton In Vitro Kit
Plasmid Kit#1000000242PurposeThe Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.DepositorAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
BIOFAB
Plasmid Kit#1000000037PurposeSets of specific constitutive bacterial promoters that vary in strength, followed by specific Bi-Cistronic Design elements varying in effectiveness to drive E. coli expression of a gene of interestDepositorAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
MetClo DNA Assembly Kit
Plasmid Kit#1000000153PurposeMetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.DepositorAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ubiquiton Human Kit
Plasmid Kit#1000000244PurposeThe Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.DepositorAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ubiquiton Yeast Kit
Plasmid Kit#1000000243PurposeThe Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.DepositorAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLIX-hN1ICD
Plasmid#91897PurposeDoxycycline inducible expression of human Notch1 ICDDepositorInsertNotch1 intracellular domain (NOTCH1 Human)
UseLentiviralTagsExpressionMammalianMutationcodons 1770 to 2555 of human NOTCH1PromoterTRE promoter, Tet ONAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR'-NRSF-CITE-GFP
Plasmid#21310DepositorInsertneuron restrictive silencer factor (Rest Mouse)
UseLentiviralTagsGFP (bicistronic with CITE/IRES)ExpressionMammalianMutationNonePromoterCMVAvailable sinceOct. 20, 2009AvailabilityAcademic Institutions and Nonprofits only -
Human ICAM-3
Plasmid#8857DepositorInsertICAM-3 (ICAM3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGL001
Plasmid#115674PurposeFluorescent reporter for E.coli K12 fliC promoter activityDepositorInsertfliC promoter fused with GFP
UseTagsGreen fluorescent protein (GFP)ExpressionBacterialMutationPromoterfliC promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Aurli_150841_GZG
Plasmid#162563PurposeInactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.DepositorInsertshble
UseUnspecifiedTagsExpressionMutationPromoterGAPDHAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
rASAP
Plasmid#178205PurposeRatiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.DepositorInsertrASAP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
rArc
Plasmid#178206PurposeRatiometric version of the genetically encoded voltage indicator ArcLight-Q239.DepositorInsertrArc
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST/LIfeAct-mCherry-N1
Plasmid#40908DepositorInsertLifeAct
UseTagsmCherryExpressionMammalianMutationNo stop codon to create c-terminal fusionPromoterCMVAvailable sinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-Flag-TRAF5
Plasmid#102679Purposeretroviral expression plasmid for TRAF5DepositorInsertTRAF5 (Traf5 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/DEST/hTfR-HA
Plasmid#69610Purposehuman transferrin receptor with an HA tag fused at the C-terminusDepositorInserthuman transferrin receptor (TFRC Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)
Plasmid#226884PurposeExpresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cellsDepositorInsertNaVAb
UseTagsMBPExpressionInsectMutationR4A, N49K, L109A, M116VPromoterPolyhedrinAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1fl
Plasmid#109157PurposeEncodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST/hCx43-EGFP-N1
Plasmid#40907DepositorInsertConnexin 43 (GJA1 Human)
UseTagsEGFPExpressionMammalianMutationNo stop codon to create c-terminal fusionPromoterCMVAvailable sinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only