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Plasmid#208807PurposeExpresses the fluorescent protein phiLOV2.1DepositorInsertphiLOV2.1
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-MagLOV
Plasmid#219957PurposeBacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2DepositorInsertMagLOV
UseTags6xHisExpressionBacterialMutationAsLOV2 C450P L496V Q513K G528K D540MPromoterT7Available sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIS8-3_DL121
Plasmid#171953PurposeExpression vector for ecDHFR-asLOV2 fusion protein. Also has TYMSDepositorInsertFusion of ecDHFR and asLOV2 at position 121 of DHFR
UseTags8-His tagExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_DL121_WTTS
Plasmid#171954PurposeSelection vector for chimeric fusion of ecDHFR and asLOV2.DepositorInsertsFusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase
UseTags8-His tagExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCri_L830-antiSH2-OptoMB-V416L
Plasmid#159936PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
UseTagsExpressionBacterialMutationAsLOV2 V416LPromoterAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCri_L889-antiSH2-OptoMB-Triple
Plasmid#159937PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
UseTagsExpressionBacterialMutationAsLOV2 V416I_G528A_N538EPromoterAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-iRIS
Plasmid#102584PurposeRegulation of protein localization (near-infrared-blue-light-inducible shuttle)DepositorInsertBphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Actin-OptoNB
Plasmid#159595PurposeOpto-NanobodyDepositorInsertActinNB with AsLOV2 at loop 5 with 62-666
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-AK74
Plasmid#159594PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at AK74
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-AK74-irFP
Plasmid#159592PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at AK74
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-GG15-irFP
Plasmid#159591PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at GG15
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-CiRISB
Plasmid#138978PurposeLight-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)DepositorInsertNcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-GG15
Plasmid#159593PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at GG15
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrc_AraC-I113-LOV
Plasmid#206804PurposeExpresses AraC-I113-LOV from a Trc promoter. For use in E. coliDepositorInsertAraC-AsLOV2 fusion
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrc_AraC-S170-LOV
Plasmid#206805PurposeExpresses AraC-S170-LOV from a Trc promoter. For use in E. coliDepositorInsertAraC-AsLOV2 fusion
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQP-NiRISB
Plasmid#138977PurposeLight-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)DepositorInsertNcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-iRISB
Plasmid#138979PurposeLight-controlled tri-directional targeting of endogenous proteinsDepositorInsertNcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)
Plasmid#203907PurposeEncodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cellsDepositorInsertGal4LOV(SK22)-VP64-IRES-mCherry
UseTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)
Plasmid#203911PurposeEncodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cellsDepositorInsertZF9LOV[C2]-VP64
UseTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)
Plasmid#203910PurposeEncodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cellsDepositorInsertZF9LOV[C1]-VP64
UseTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only