1. pLKO.1 puro

   Plasmid

   #8453

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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1. pCE-K2N

   Plasmid

   #154879

   Purpose

   Episomally expresses KLF2 and NANOG in mammalian cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

   Insert

   KLF2-2A-NANOG (KLF2, NANOG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPBCAG-rtTAM2-2A-SOX17-GR-IH

   Plasmid

   #165079

   Purpose

   Ubiquitously expresses rtTAM2, dexamethasone-inducible human SOX17, and hygromycin-resistance gene.

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

   Inserts

   rtTA-M2
   SOX17 (SOX17 Human)
   

   Use

   Piggybac transposition

   Tags

   Glucocorticoid Receptor

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCE-KdGl

   Plasmid

   #154880

   Purpose

   Episomally expresses KDM4D and GLIS1 in mammalian cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

   Insert

   KDM4D, GLIS1 (KDM4D, GLIS1 Human, Callithrix jacchus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR221_FLVCR1

   Plasmid

   #131892

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   FLVCR1 (FLVCR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pWZL Neo Myr Flag ADCK4

   Plasmid

   #20544

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   ADCK4 (ADCK4 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pDONR221-FLVCR1_STOP

   Plasmid

   #161058

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   FLVCR1 (FLVCR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pDONR223-DYRK2

   Plasmid

   #23690

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   DYRK2 (DYRK2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pWZL Neo Myr Flag DYRK2

   Plasmid

   #20479

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   DYRK2 (DYRK2 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pDONR223-ADCK4

   Plasmid

   #23711

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ADCK4 (ADCK4 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. Ekker Lab TALEN kit

    Plasmid Kit

    #1000000038

    Purpose

    Contains all 256 possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs for high-throughput mutagenesis

    Depositor

    Stephen Ekker

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only