1. pLKO.1 puro

   Plasmid

   #8453

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pUC118-FLIP-FlpE-Puro

   Plasmid

   #84539

   Purpose

   a mock vector for generating FLIP-FlpE targeting vectors

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   FLIP-FlpE - Puro
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC118-FLIP-Puro

   Plasmid

   #84538

   Purpose

   a mock vector for generating FLIP targeting vectors

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   FLIP - Puro
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pAG415-ADH1p-HA-rap1-K9R

   Plasmid

   #84531

   Purpose

   This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   ADH1

   Available since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pAG415-GPDp-EGFP-rap1-K3NR

   Plasmid

   #84533

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(43,117,118)R

   Promoter

   GPD

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pAG415-GPDp-EGFP-rap1-K3CR

   Plasmid

   #84534

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(462,463,651)R

   Promoter

   GPD

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAG415-GPDp-EGFP-rap1-K9R

   Plasmid

   #84535

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   GPD

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAG415-GPDp-EGFP-rap1-K651R

   Plasmid

   #84536

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K651R

   Promoter

   GPD

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

8. crTet in pSLQ8453

   Plasmid

   #183965

   Purpose

   U6-crTet-EF1a-Puro-T2A-BFP-WPRE

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   crTet
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CLP1 gRNA (BRDN0001148453)

   Plasmid

   #76144

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CLP1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CLP1 (CLP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

    Plasmid

    #140086

    Purpose

    Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    LbCpf1 (LbCas12a) crRNA backbone
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-DN-hCUL2-FLAG

    Plasmid

    #15819

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005 . 399():287-309.

    Insert

    Cullin2 (CUL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N427

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-myc3-CUL2

    Plasmid

    #19892

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 2 (CUL2 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 3xFLAG-CUL2-pCMV7.1

    Plasmid

    #155020

    Purpose

    N-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 2 (CUL2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE DN Cullin2

    Plasmid

    #41912

    Depositor

    Stephen Elledge

    Article

    Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

    Insert

    CUL2 (CUL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac Dual His6-TEV-CUL2, Rbx1

    Plasmid

    #231000

    Purpose

    Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Inserts

    Rbx1 (Rbx1 Mouse)
    CUL2 (Cul2 Mouse)
    

    Use

    Tags

    6x HIS

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1 GFP shRNA

    Plasmid

    #30323

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    GFP shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1-puro-shNT

    Plasmid

    #109012

    Purpose

    short-hairpin knockdown of targeted gene

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting shRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.1-puro-shFAM134B

    Plasmid

    #109013

    Purpose

    short-hairpin knockdown of targeted gene

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    shFAM134B (RETREG1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1 RagC shRNA #2

    Plasmid

    #26630

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagC shRNA (RRAGC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only