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Plasmid#198476Purposelentiviral stable expression of mRab12 gRNA 2DepositorInsertmouse Rab12 (Rab12 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mRab12-1
Plasmid#198475Purposelentiviral stable expression of mRab12 gRNA 1DepositorInsertmouse Rab12 (Rab12 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3HA-hMIB1
Plasmid#33317DepositorInsertMIB1 (MIB1 Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-mCherry-RUSH
Plasmid#202799PurposeRUSH cargo: Str-KDEL_IRES_SBP-mCherry-Lamp1DepositorInsertLamp1 (Lamp1 Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT WT (Puro)
Plasmid#154290PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationWTPromoterPGKAvailable sinceDec. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT K842M
Plasmid#154284PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationK842M mutation (Catalytic Inactive)PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A mKate2-hu_ncOGT D554N
Plasmid#154283PurposeEntry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationD554N mutation (Ser/Thr Glycosylation Impaired)PromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT K842M
Plasmid#154272PurposeBacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationLysine 842 changed to Methionine- bacterial codon…PromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT D554N
Plasmid#154279PurposeBacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationAspartate 554 changed to Asparagine- bacterial co…PromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3F-Strep-hMIB2
Plasmid#33313DepositorInsertMIB2 (MIB2 Human)
UseTags3xFlag and StrepOneExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D RNAiR
Plasmid#51600Purposeexpresses RNAi-resistant mouse Sema4D with N-terminal myc tagDepositorInsertSema4D (Sema4d Mouse)
UseTagsmycExpressionMammalianMutationsilent mutations between amino acids (approx 275-…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-Δ37, 115-133, 204-217 PPM1H
Plasmid#207344PurposeMammalian expression of HA-Δ37, 115-133, 204-217 PPM1HDepositorInsertPPM1H (PPM1H Human)
UseTagsHAExpressionMammalianMutationΔ37, Δ115-133, Δ204-217PromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mCsnk2b - 1
Plasmid#198505Purposelentiviral stable expression of mCsnk2b gRNA 1DepositorInsertmCsnk2b (Csnk2b Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mCsnk2b - 2
Plasmid#198506Purposelentiviral stable expression of mCsnk2b gRNA 2DepositorInsertmCsnk2b (Csnk2b Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mMyh9 - 2
Plasmid#198492Purposelentiviral stable expression of mMyh9 gRNA 2DepositorInsertmMyh9 (Myh9 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-guide-puro mMyh9 - 1
Plasmid#198491Purposelentiviral stable expression of mMyh9 gRNA 1DepositorInsertmMyh9 (Myh9 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Antibody#184214-rAbPurposeAnti-Mitofusin-1 (Mouse) recombinant mouse monoclonal antibodyDepositorRecommended ApplicationsImmunocytochemistryReactivityMouse and RatSource SpeciesMouseIsotypeIgG2aTrial SizeAvailable to purchaseAvailable sinceAug. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits
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CD-MPR-mCherry-RUSH
Plasmid#202798PurposeRUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPRDepositorInsertM6PR (M6PR Human)
UseTagsmCherryExpressionMammalianMutationChanged 40 K (AAA) with R (AGA)PromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
CD-MPR-GFP-RUSH
Plasmid#202797PurposeRUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPRDepositorInsertM6PR (M6PR Human)
UseTagsEGFPExpressionMammalianMutationChanged 40 K (AAA) with R (AGA)PromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK FKBP12(F36V)-OGT (Neo)
Plasmid#154294PurposeLentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoterDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x HA and FKBP12(F36V)ExpressionMammalianMutationPromoterPGKAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only