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Plasmid#118998PurposeMammalian expression of Rosa26 sgRNA, Cas9 and BFPDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TetR-RNF169UBD
Plasmid#119011PurposeMammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expressionDepositorInsertTetR
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dox-SOScat
Plasmid#207329PurposePiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)DepositorInsertson of sevenless homolog 2 variant, catalytic domain
UseTagsmyristoylation tag - BFPExpressionBacterialMutationrange: 593 to 1078PromoterTet Response ElementAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Rad18UBD
Plasmid#119005PurposeMammalian expression of the human Rad18 Ubiquitin binding domain and BFPDepositorInsertRad18
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-RNF169UBD
Plasmid#119006PurposeMammalian expression of the human RNF169 Ubiquitin binding domain and BFPDepositorInsertRNF169 (RNF169 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-BbTagBY (AAV9)
Viral Prep#45185-AAV9PurposeReady-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsTagBFP, EYFP, or none.Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA
Plasmid#138271PurposeDual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)DepositorInsertsgBG, sgTS
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW842_pCAG-pExpr-FP-DECODER
Plasmid#87551PurposeExpresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinasesDepositorInsertmtagBFP, EGFP, iRFP720, mRuby2
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNR291
Plasmid#79555PurposeOutput plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.DepositorInsertsGFPmut3b
mTagBFP
mRFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO
Plasmid#68405PurposeThe prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoterDepositorInserts3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
FlpO
UseCRISPRTagsmTurquoise2 (BFP)ExpressionMammalianMutationPromoterSynthetic Probasin ARRx2 and U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti-gRNA
Plasmid#226867PurposeLentiviral expression of Sp-gRNA with BlpI and BstXI restriction sites for gRNA spacer cloning. Also expresses BFP.DepositorInsertLenti Sp-gRNA
UseLentiviralTagsExpressionMutationPromotermU6Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo41
Plasmid#204016PurposeCloning plasmid containing the BFP insert with a chloramphenicol resistance markerDepositorInsertBlue fluorescent protein
UseTagsExpressionPlantMutationPromoterN/AAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
InterTag
Plasmid#186910PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
InterTag-3xL-Δtag
Plasmid#186914PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, expressing sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side BFP and puromycin resistance geneDepositorInsertsfCherry11-(GGGGS)3-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
IDP378
Plasmid#87005Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertpreLib-bfp-4xboxB
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC025
Plasmid#227597PurposeLentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motifDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMN-009
Plasmid#195413PurposeAAVS1 Integratable mCherry Reporter for synZiFTR ZF1DepositorInsert4X ZF1 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC026
Plasmid#227598PurposeLentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-QtgRNA2.1-BR
Plasmid#170513PurposegRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6:3Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
InterTag-3xL
Plasmid#186913PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance geneDepositorInsertSpyTag003-sfCherry11-(GGGGS)3-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only