We narrowed to 3,027 results for: BFP

1. pJT244 Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast

   Plasmid

   #195537

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV)

   Plasmid

   #107860

   Purpose

   To quickly insert CTD mutants into TDP43 splicing reporter

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of CTD in TDP43 splicing reporter, inser…

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pHBS1333 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 G368W W385G)

   Plasmid

   #107855

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   G368W and W385G in splicing reporter

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

   Plasmid

   #229814

   Purpose

   A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   loxp-EGFP-stop-lox
   

   Use

   Cre/Lox; Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #176273

   Purpose

   Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (sgTet) and BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #176272

   Purpose

   Expression of non-targeting sgRNA of CasMINI

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI non-targeting sgRNA (sgLacZ) and BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT337 Lenti pEF-dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast

   Plasmid

   #187341

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-Rox-nlsKate5V-stop-Rox-myr-Flag-BFP-NEO (JDW 473)

   Plasmid

   #242587

   Purpose

   A CAGGS driven, Dre recombinase dependent switch reporter (nls-mKate2 to MbBFP following Dre recombination).

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   nlsKateV5, MbBPF-FLAG, FRTNeoFRT
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

   Plasmid

   #229816

   Purpose

   A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   loxp-EGFP-stop-lox
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

    Plasmid

    #229841

    Purpose

    A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

    Depositor

    Joshua Wythe

    Article

    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

    Insert

    loxp-H2B-mCerulean-2xStop-loxP
    

    Use

    Cre/Lox; Tol2 based expression vector

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT241 Lenti pEF-dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast

    Plasmid

    #187326

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT342 Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

    Plasmid

    #195541

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pW299-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-tagBFP-P2A-BlastR

    Plasmid

    #189943

    Purpose

    Lentiviral vector to co-express an spsgRNA with NLS-tagBFP

    Depositor

    Kenneth Yamada

    Article

    Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9

    Plasmid

    #84242

    Purpose

    Expresses GA-inducible KRAB-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    GID1-KRAB
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC

    Plasmid

    #135465

    Purpose

    Membrane-anchored TF used for yeast directed evolution of TEV protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
    

    Tags

    BFP, flag

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid

    #84240

    Purpose

    Expresses GA-inducible VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid

    #84244

    Purpose

    Expresses GA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sa dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid

    #140228

    Purpose

    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    RRM2 endogenous promoter

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRCA893 - pBA904 Puro-T2A-BFP KLF5 g1 CRISPRa guide guide (pRCA594 backbone) 666

    Plasmid

    #238186

    Purpose

    Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF5 sgRNA CRISPRa (KLF5 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    mTagBFP2

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRCA895 - pBA904 Puro-T2A-BFP KLF4 g1 CRISPRa guide guide (pRCA594 backbone) 668

    Plasmid

    #238187

    Purpose

    Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 sgRNA CRISPRa (KLF4 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    mTagBFP2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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