Addgene

We narrowed to 2,259 results for: 828

Showing: 1561 - 1580 of 2259 results

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pDEST-EGFP-MACROD1

Plasmid

#172584

Purpose

For transient expression of N-terminal GFP-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-MACROD2

Plasmid

#172585

Purpose

For transient expression of N-terminal GFP-tagged MACROD2

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK614

Plasmid

#110608

Purpose

BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

dCas9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-CBX7 Chromo

Plasmid

#59695

Purpose

Contains histone modification interacting domain (CBX7 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

CBX7 Chromo (CBX7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCVneo-HOXB3-FLAG

Plasmid

#74508

Purpose

Retroviral expression of HOXB3

Depositor

Lars Rönnstrand

Article

Lindblad et al Biochem Biophys Res Commun. 2015 Nov 27;

Insert

HOXB3 (HOXB3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

unknown

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

Plasmid

#83461

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, R4113A

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBTK601

Plasmid

#110607

Purpose

BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Cas9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_1

Plasmid

#103919

Purpose

Protein expression and purification of PTN13 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_3

Plasmid

#103921

Purpose

Protein expression and purification of PTN13 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX2T-p19Ink4d

Plasmid

#52222

Purpose

bacterial expression of p19Ink4d

Depositor

Martine Roussel

Article

Forget et al Cell Cycle. 2008 Dec;7(23):3737-46. Epub

Insert

Ink4d (Cdkn2d Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only
pBI101-pGC1(D1)-GUS

Plasmid

#49513

Purpose

Expresses beta-glucuronidase gene under the control of guard cell promoter (pGC1 (D1)) in plant

Depositor

Julian Schroeder

Article

Yang et al Plant Methods. 2008 Feb 19;4:6. doi: 10.

Insert

Promoter of GC1(D1) (AT1G22690 Mustard Weed)

Use

Plant expression

Tags

beta-glucuronidase

Expression

Mutation

Promoter

GC1 (At1g22690), 1141 bp upstream of the transcri…

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
PTPN13_PDZ_2

Plasmid

#103920

Purpose

Protein expression and purification of PTN13 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_5

Plasmid

#103923

Purpose

Protein expression and purification of PTN13 PDZ5 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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N-TAP A2A in pcDNA3

Plasmid

#67850

Purpose

mammalian expression of A2A receptor with N terminal TAP tag

Depositor

Christian Gruber

Article

Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

Insert

adenosine A2a receptor (ADORA2A Human)

Use

Tags

TAP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
Frt-hspB10

Plasmid

#63101

Purpose

Expression of HSPB10 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb10 (ODF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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Cs2+Etv5

Plasmid

#49095

Purpose

expression of Etv5

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv5 (etv5a Zebrafish)

Use

Xenopus/avian/zebrafish

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pFOSGE_DUM1H01

Plasmid

#71090

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

nej (nej Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
pMH-HT_SsCHAD-H29A-R32A-R36A-R69A

Plasmid

#127787

Purpose

Expresses a SsCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

mutations in His29, Arg32, Arg36 and Arg69 to Ala

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNAFut9

Plasmid

#161021

Purpose

a fucosyltransferase 9 (Fut9) expression plasmid, containing full-length mouse Fut9 cloned into pcDNA3.1+

Depositor

Andreas Faissner

Article

Hennen et al J Biol Chem. 2011 May 6;286(18):16321-31

Insert

Fucosyltransferase 9 (Fut9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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R9-WT

Plasmid

#125210

Purpose

Expressing wildtype human Rad9b gene

Depositor

Richard Finnell

Article

Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

Insert

Rad9b (RAD9B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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