pcDNAFut9
(Plasmid #161021)

• Purpose: a fucosyltransferase 9 (Fut9) expression plasmid, containing full-length mouse Fut9 cloned into pcDNA3.1+
• Depositing Lab: Andreas Faissner
• Publication: Hennen et al J Biol Chem. 2011 May 6;286(18):16321-31. doi: 10.1074/jbc.M110.201095. Epub 2011 Mar 8.

Backbone

• Vector backbone: pcDNA3.1+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6513
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Fucosyltransferase 9
• Alt name: Fut9
• Species: M. musculus (mouse)
• Insert Size (bp): 1085
• Entrez Gene: Fut9 (a.k.a. AI746471, AU067636, mFUT9, mFuc-TI, mFuc-TIX)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GGATCCATGACATCAACATCCAAAGGC
• 3′ sequencing primer: GAATTCTTAATTCCAAAACCATTTCTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Eva Hennen cloned full-length mouse Fut9 into the pcDNA3.1+ vector which was obtained from Invitrogen.