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pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta

Plasmid

#79913

Purpose

piggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Zeocin-t2A-sHRPbNRX3beta

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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peSpdCas9-J23109

Plasmid

#113153

Purpose

Plasmid constitutively expressing eSpdCas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

eSpdCas9

Use

Tags

Expression

Bacterial

Mutation

D10A & K810A & H840A & K1003A & R…

Promoter

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pNAS1B split mCherry N-MESVD/TRAP(RK)-C

Plasmid

#61967

Purpose

Duet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-MESVD
TRAP(RK)-C-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD and PLtetO

Available since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pISH-Prrx1

Plasmid

#84322

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Prrx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Prrx1 (Prrx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pUASt-ssBiP-ESCargo

Plasmid

#140166

Purpose

Expresses a regulatable secretory cargo for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Insect

Mutation

Promoter

hs70

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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mCitrine-USP19 1-1290

Plasmid

#78595

Purpose

Express mCitrine-USP19 1-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 1-1290 (USP19 Human)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-Scs2

Plasmid

#58834

Purpose

Expresses GFP-Tagged Scs2 in yeast

Depositor

Tim Levine

Article

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Scs2 (SCS2 Budding Yeast)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pAM4645

Plasmid

#85124

Purpose

NSII vector expressing PkaiB-KaiBC. Nt resistant. Complements rhthyms in AMC705

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

PkaiBC-KaiBC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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Lifeact-rsLOV1 pcDNA3.1(+)

Plasmid

#107881

Purpose

Mammalian expression of Lifeact-rsLOV1

Depositor

Stefan Hell

Article

Gregor et al Sci Rep. 2018 Feb 9;8(1):2724. doi: 10.1

Insert

rsLOV1

Use

Tags

Lifeact

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCSDC2.1.0-gDNA

Plasmid

#113781

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CSDC2

Depositor

Kevin White

Insert

CSDC2 (CSDC2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Pou2f3

Plasmid

#84321

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pou2f3 (Pou2f3 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBlue-hFX(I154F)(AA88)-HA

Plasmid

#15403

Depositor

Michael Ristow

Insert

human frataxin cDNA (FXN Human)

Use

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
hSDH5_G78R

Plasmid

#113047

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_G78R (SDHAF2 Human)

Use

Tags

Expression

Bacterial

Mutation

G78R, Insert begins at amino acid 39

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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hP14H8y

Plasmid

#84368

Purpose

antigen for human PRDM14 purification ( aa 1-243)

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

contains PRDM14 AA1-243

Promoter

T7

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-YC

Plasmid

#107072

Purpose

Host vector to construct a YPer or CyPet fusion protein

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

YPet-CyPet fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Emx1

Plasmid

#84309

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Emx1 (Emx1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTG005

Plasmid

#71386

Purpose

localization of yeast RNase RMP1 (RNase MRP binding protein) in yeast

Depositor

Mark Schmitt

Article

Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

Insert

RMP1 (RMP1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

ACT1

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBluescript-KS-Nrg3-ISH

Plasmid

#87669

Purpose

In situ probe for mouse Nrg3

Depositor

Oscar Marin

Article

Bartolini et al Cell Rep. 2017 Jan 31;18(5):1157-1170. d

Insert

Nrg3 (Nrg3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7/M13

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG

Plasmid

#91929

Purpose

Bacterial expression of maize SUMO1a

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Insert

6His-ZmSUMO1a-GG

Use

Tags

6His

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Tshz3

Plasmid

#74362

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

eashirt zinc finger family member 3 (Tshz3 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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