Addgene

We narrowed to 11,119 results for: 110

Showing: 1561 - 1580 of 11119 results

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pmNeonGreenHO-3xFLAG-G

Plasmid

#127914

Purpose

a mammalian expression backbone vector for humanized mNeonGreen-3xFlag

Depositor

Isei Tanida

Article

Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

Insert

humanized mNeonGreen

Use

Tags

mNeonGreen-3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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AByG

Plasmid

#111083

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

3xP3 promoter
tdTomato
Gypsy insulator
CTCF insulator
Y homology arm left
Y homology arm right

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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VV001: WT Arch-3 in pET28b

Plasmid

#58487

Purpose

Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3 (with C-terminal His tag)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pSB3C5-phzSM

Plasmid

#141105

Purpose

Contains a phzS-phzM operon BioBrick

Depositor

Martin Buck

Article

Clifford et al Chem Sci. 2021 Jan 15;12(9):3328-3338. d

Insert

phzSM

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-phzAG

Plasmid

#141106

Purpose

Contains a seven gene phzABCDEFG operon BioBrick

Depositor

Martin Buck

Article

Clifford et al Chem Sci. 2021 Jan 15;12(9):3328-3338. d

Insert

phzABCDEFG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Filamin-M-stFRET

Plasmid

#61105

Purpose

Expression of Filamin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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Actinin-M-stFRET

Plasmid

#61103

Purpose

Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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Actinin-C-stFRET

Plasmid

#61104

Purpose

Expression of α-actinin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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AByF

Plasmid

#111084

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

Y homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJBM1

Plasmid

#225288

Purpose

E. coli- C. coli shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pWestmill 11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046482

Plasmid

#149110

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5_FRT_GFP_6TS_mCH

Plasmid

#127110

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

GFP-mCherry fusion

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN51 (A167R)

Plasmid

#80110

Purpose

Late1-O3-33(A167R)-myc-PH

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51 (A167R)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pre-associated oBC-minP-GFP-mBC backbone (p043)

Pooled Library

#194098

Purpose

pre-associated oBC-minP-GFP-mBC backbone (p043), can directly be used to deposit library of regulatory elements

Depositor

Jay Shendure

Article

Lalanne et al bioRxiv 2022.12.10.519236

Expression

Bacterial and Mammalian

Use

RNAi

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only
scAAV-hIBA1a-GFP

Plasmid

#214146

Purpose

Self-complementary AAV vector packaging GFP under the human Iba1 promoter

Depositor

Chun-Li Zhang

Article

Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XHA-EGFP-PEX26

Plasmid

#139054

Purpose

HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XHA-EGFP-PEX26

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

UBC

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-GFP1-10

Plasmid

#80409

Purpose

Expresses GFP1-10 in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP1-10

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSB19-PapRS#34-OmpA

Plasmid

#231105

Purpose

Vector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)

Depositor

Arne Skerra

Article

Mayrhofer et al Nat Commun. 2024 Dec 18;15(1):10693. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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VPS13D^mScarlet

Plasmid

#174110

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Use

Tags

mScarlet

Expression

Mammalian

Mutation

With an internal mScarlet tag in aminoacid positi…

Promoter

CMV

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCR1265

Plasmid

#111094

Purpose

lentiviral cDNA expression vector: plenti-x1-neo-linker-IRES-Thy1.1

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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