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Plasmid#216837PurposeFor mammalian expression of NAP1-GFP (Internal ref. SMC1638)DepositorInsertNAP1
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Q443P
Plasmid#216401PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutationDepositorInsertEfg1-ΔDBD-Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Glutamine 443 to Proline, and deleted ami…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE4 CTFdeltaTA-FLAG
Plasmid#217725PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE4
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-179PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template
Plasmid#215000PurposeEndogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.DepositorInsertmsTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-TrxF
Plasmid#207454PurposeE. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tagDepositorInsertThioredoxin F
UseTags6xHisExpressionBacterialMutationCodon optimized for E coli expressionPromoterT7Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2
Plasmid#210749PurposeCoding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeatsDepositorInsertsCjSp D8A Cas9
Bilbo sgCAG
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationD8A, N-terminal Cj Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-V44D/V314D-mCherry-Hygro
Plasmid#210855PurposeLentiviral expression vector encoding FIP200 mutant-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationV44D/V314DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-V314D-mCherry-Hygro
Plasmid#210854PurposeLentiviral expression vector encoding FIP200 mutant-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationV314DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-V44D-mCherry-Hygro
Plasmid#210853PurposeLentiviral expression vector encoding FIP200 mutant-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationV44DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-ATG101
Plasmid#210858PurposeMammalian expression of ATG101DepositorInsertATG101 (ATG101 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF
Plasmid#210842PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationL324D/R543D/R548DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 AvrRpm1
Plasmid#209185PurposepENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tagsDepositorInsertAvrRpm1
UseGateway-compatible entry vectorTagsExpressionMutationPromoternoneAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mNeon-Zeo
Plasmid#207731PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Zeo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-miRFP670nano3-Zeo
Plasmid#207735PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Zeo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-3xFLAG-Zeo
Plasmid#207739PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-3xHA-Zeo
Plasmid#207740PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-miRFP670nano3-Blast
Plasmid#207713PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-V5-Blast
Plasmid#207719PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-miRFP670nano3-Puro
Plasmid#207724PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Zeo-mNeon
Plasmid#207698PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mNeon cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only