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Plasmid#163864PurposesgRNA targeting GFP in C. elegans. Backbone: pJJR50.DepositorInsertsgRNA targeting GFP
UseCRISPRTagsExpressionWormMutationPromoterR07E5.16 (U6)Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1H5
Plasmid#165065PurposeCharacterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPY2A
Plasmid#165066PurposeCharacterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFPDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>Y-FAST-ErB1.2A-yEGFP>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP105
Plasmid#165060PurposeIntegrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP104
Plasmid#165059PurposeIntegrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54_mEGFP494(-6)
Plasmid#163434PurposeMammalian expression of Nup54 with a mEGFP (missing the first 6 amino acids) at amino acid position 494DepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup133_mEGFP(-9b)
Plasmid#163420PurposeMammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)DepositorInsertNup133 (NUP133 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup133_mEGFP(-8b)
Plasmid#163418PurposeMammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)DepositorInsertNup133 (NUP133 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBz172_CsoS2_N1_GFP
Plasmid#140852PurposeHis-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backboneDepositorInsertCsoS2 NTD repeat 1 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz33_CsoS2_NTD_GFP
Plasmid#140861PurposeHis-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFPDepositorInsertCsoS2 NTD fused to GFP
UseTagsHexahistidine tag and Superfolder GFPExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz175_CsoS2_N4_GFP
Plasmid#140855PurposeHis-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 4 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz173_CsoS2_N2_GFP
Plasmid#140853PurposeHis-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 2 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz174_CsoS2_N3_GFP
Plasmid#140854PurposeHis-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backboneDepositorInsertCsoS2 NTD repeat 3 fused to GFP
UseTagsHexahistidine tag, Strep-Tag II, and Superfolder …ExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Unc104_K560GFP
Plasmid#129773PurposeExpresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertUnc104 (unc-104 Nematode)
UseTagsGFP and His6ExpressionBacterialMutationTruncation at 361, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_UNK_G1
Plasmid#127118DepositorInsertgRNA UNK (UNK Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5_FRT_GFP_12AAA_mCH
Plasmid#127111DepositorInsertGFP-mCherry fusion
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5_FRT_GFP_6TS_mCH
Plasmid#127110DepositorInsertGFP-mCherry fusion
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV.UBI.GFP.miR292T
Plasmid#115972PurposemiR-292 regulated GFP expressionDepositorInsertGFP.miR292T
UseLentiviralTagsGFPExpressionMutationPromoterubiquitinAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW8_yEGFP
Plasmid#110229PurposeCre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent proteinDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT25
Plasmid#89664Purposeexpression of doubleTAL staple protein dT25 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT25
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only