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Plasmid#145863PurposeInsect Expression of CG7785-3UTRDepositorInsertCG7785-3UTR (CG7785 Fly)
UseTagsExpressionInsectMutation317bp deletion in 3'UTR region (4172-4273bp)…PromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmDCP2_209-791_A
Plasmid#145848PurposeInsect Expression of DmDCP2_209-791DepositorInsertDmDCP2_209-791 (DCP2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-Teasy-Exp
Plasmid#66791PurposeExpression of D. melanogaster CG13188DepositorInsertCG13188 (CG13188 Fly)
UseTagsExpressionBacterialMutationH452N (see depositor's comments below)PromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRD65 (dDP)
Plasmid#14821DepositorInsertDP (Dp Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pWalium20-10XUAS-3XFLAG-dCas9-VPR
Plasmid#78897PurposeExpresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRaDepositorInsertdCas9-VPR
UseTags3xFLAGExpressionInsectMutationPromoter10XUASAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3XP3.RFP-tan
Plasmid#83474PurposeRFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsert3XP3.RFP-tan
UseCRISPRTagsExpressionBacterial and InsectMutationPromoter3XP3 eye-expression PromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCFD3-frame_selector_2
Plasmid#127555PurposeDrosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS-syb:spGFP1–10
Plasmid#73698PurposeNeuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASPDepositorInsertNeuronal-synaptobrevin:spGFP1–10 chimera
UseDrosophilaTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B
Plasmid#21682DepositorInsertmaternal expression at 31B (me31B Fly)
UseDrosophila expressionTagsEGFPExpressionMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAct-PE2-HygroR
Plasmid#149552PurposeExpression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.DepositorInsertPE2
UseTagsExpressionInsectMutationPromoterAct5cAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pActP-dtrpA1
Plasmid#64713Purposeinducing calcium influx in cultured Drosophila S2 cellsDepositorInsertdtrpA1 (TrpA1 )
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
tubP-FRT>GAL80-6-FRT>
Plasmid#63173Purposeubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassetteDepositorInsert"Flp-Out" GAL80
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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UAS-Syt::7xHalo7
Plasmid#87650PurposeFor creating drosophila transgenics expressing UAS-Syt::7xHalo7DepositorInsertSyt::7xHalo7
UseTagsExpressionInsectMutationS265G in Syt (please see depositor's commen…PromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::Halo7
Plasmid#87648PurposeFor creating drosophila transgenics expressing UAS-Syt::Halo7DepositorInsertSyt::Halo7
UseTagsExpressionInsectMutationS265G in Syt (please see depositor's commen…PromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
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