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pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

Plasmid

#51257

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248,789,805,817,848,9…

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pJEP304-pAAV-EFS-dSaCas9-VP64-pA

Plasmid

#113679

Purpose

A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV, CRISPR, and Synthetic Biology

Tags

NLS and VP64

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

Plasmid

#51256

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 216,220,224,230,248 mutated to Glu

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-CATULIN

Plasmid

#118979

Purpose

Expresses full length of Catulin-GFP (Mus musculus). Contains CMV and T3 promoter for mammalian expression and mRNA production.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Alpha Catulin (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF0147

Plasmid

#142464

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SNW1 (SNW1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-CBX1 Chromo

Plasmid

#59697

Purpose

Contains histone modification interacting domain (CBX1 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

CBX1 Chromo (CBX1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 P1-4A

Plasmid

#51252

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 789,805,817,848 mutated to Ala

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
Rat SERCA2b (pMT2)

Plasmid

#75184

Purpose

Mammalian expression of ATP2A2

Depositor

Gary Shull, Jonathan Lytton

Article

Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

Insert

ATP2A2

Use

Tags

Expression

Mammalian

Mutation

Promoter

adenovirus major late promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-4

Plasmid

#112844

Purpose

transient/stable overexpression of the N-terminal and central domains of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

N + C1 domains of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 P5-6D

Plasmid

#51254

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 923,956 mutated to Asp

Promoter

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pAPM-D4 miR30-AGO1 ts1

Plasmid

#115850

Purpose

AGO1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

AGO1 shRNA (AGO1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-AGO1 ts3

Plasmid

#115847

Purpose

AGO1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

AGO1 shRNA (AGO1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2

Plasmid

#112842

Purpose

transient/stable overexpression of the central domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

C1 domain of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 P5-6A

Plasmid

#51253

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 923,956 mutated to Ala

Promoter

EF1a

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
Gal4-VP16-HSF1Δ∼PRD

Plasmid

#71727

Purpose

plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Human, Herpes Simplex Virus protein VP16, Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_Zeo_TetO_FLAG-AT1R

Plasmid

#222856

Purpose

Mammalian expression plasmid for FLAG-tagged AT1R

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT1R (AGTR1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-MIR302-7guides-PGK-Puro

Plasmid

#201960

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

Depositor

Timo Otonkoski

Article

Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

Insert

MIR302-7g-PGK-Puro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-Halo-Dync1h1motor

Plasmid

#191333

Purpose

Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

DYNC1H1 motor domain aa 1453-4644

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-Dync1h1motor

Plasmid

#191332

Purpose

Expresses the dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-Dync1h1motor (Dync1h1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

DYNC1H1 motor domain aa 1453-4644

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-VP16

Plasmid

#183927

Purpose

Optogenetic PHR domain coupled to GFP and VP16 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-VP16 (CRY2, UL48 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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