-
Plasmid#148859PurposeMammalian Expression of HsXRN1-sgRNADepositorInsertHsXRN1-sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH
Plasmid#148857PurposeMammalian Expression of HsPatL1-sgRNADepositorInsertHsPatL1-sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH
Plasmid#148858PurposeMammalian Expression of HsTNRc6C-sgRNADepositorInsertHsTNRc6C-sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_4
Plasmid#192688PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_1
Plasmid#192685PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_2
Plasmid#192686PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_3
Plasmid#192687PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJR98
Plasmid#187239PurposeCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sitesDepositorInsertCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP2
Plasmid#79874PurposeBacillus subtilis sgRNA expression vector; integrates into amyEDepositorInsertsgRNA RR1
UseCRISPRTagsExpressionBacterialMutationPromotervegAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRTagsExpressionBacterialMutationPromotervegAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_ACTB CRISPIE
Plasmid#172853PurposeActb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.DepositorInsertsActb intron 1 sgRNA
DRS-2 sgRNA
CRISPIE designer exon (phase 0-0) encoding mEGFP
mRuby3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD5_w
Plasmid#112645PurposeUbiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.DepositorInsertU6:3-tRNA-sgRNA expression cassette
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pDECKO_mCherry_AAVS1
Plasmid#141212PurposepDECKO_mCherry_AAVS1_186. Targeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRNA telo/GFP
Plasmid#137713PurposeVector co-expressing sgRNA telo and GFPDepositorInsertsgRNA telo + GFP
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6/RSVAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_GFP_AAVS1_186
Plasmid#159091Purposetargeting, non-essential locus controlDepositorInsertgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMZ374
Plasmid#59896PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
phU6 ST
Plasmid#188712PurposesgRNA plasmid encoding hU6 promoter driving an anti-safe targeting sgRNADepositorInsertSafe Targeting sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmU6 ST
Plasmid#188713PurposesgRNA plasmid encoding mU6 promoter driving an anti-safe targeting sgRNADepositorInsertSafe Targeting sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p7SK ST
Plasmid#188714PurposesgRNA plasmid encoding 7SK promoter driving an anti-safe targeting sgRNADepositorInsertSafe Targeting sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only