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Plasmid#101220PurposeBacterial expression plasmid for SpCas9 Cluster 1 + Q926A variantDepositorInsertSpCas9 variant N692A/M694A/Q695A/H698A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationN692A, M694A, Q695A, H698A and Q926APromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant
Plasmid#101232PurposeBacterial expression plasmid for SpCas9 Cluster 2 + Q926A variantDepositorInsertSpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationG528A, V583A, E584A, D585A, N588A and Q926APromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-hDcr-K70A.
Plasmid#89145PurposeExpression of helicase mutant of human Dicer in Sf9 cellsDepositorInserthDicer (DICER1 Human)
UseTagsHis6-tevExpressionInsectMutationK70APromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-Gja1prom
Plasmid#188114PurposeExpresses luciferase under control of Gja1 promoter in transfected cellsDepositorInsertGja1 promoter (Gja1 Mouse)
UseLuciferaseTagsExpressionMutationPromoterGja1 endogenous promoter up to gene start codonAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAT079
Plasmid#180509PurposePlasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
DpbCasX
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT077
Plasmid#180511PurposePlasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
PlmCasX
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_163 SpCas9 K233A/K234A/K253A/K263A
Plasmid#179527PurposeFor bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 K233A/K234A/K253A/K263A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged lysines 233, 234, 253, and 263 to alaninePromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant
Plasmid#101207PurposeBacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variantDepositorInsertSpCas9 variant N497A/R661A/Q695A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationN497A, R661A and Q695APromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant
Plasmid#101201PurposeBacterial expression plasmid for SpCas9, REC3 FRET variantDepositorInsertSpCas9 variant C80S/C574S/S701C/S960C
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S701C and S960CPromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant
Plasmid#101214PurposeBacterial expression plasmid for SpCas9 + K855A, HNH FRET variantDepositorInsertSpCas9 variant C80S/C574S/S355C/S867C/K855A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S355C, S867C and K855APromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant
Plasmid#101200PurposeBacterial expression plasmid for SpCas9, REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C and D273CPromoterT7Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK4387 - human expression plasmid for SpCas9 Cluster 5
Plasmid#101191PurposeHuman expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster5(K918A/V922A/R925A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationK918A/V922A/R925APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3645 - human expression plasmid for SpCas9 Cluster 3
Plasmid#101187PurposeHuman expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster3(T657A/G658A/W659A/R661A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationT657A/G658A/W659A/R661APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3770 - human expression plasmid for SpCas9 Cluster 4
Plasmid#101189PurposeHuman expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster4(F491A/M495A/T496A/N497A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationF491A/M495A/T496A/N497APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW2993 - human expression plasmid for SpCas9 Cluster 2
Plasmid#101185PurposeHuman expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationG582A/V583A/E584A/D585A/N588APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-EF1a-D614G-N501Y
Plasmid#177939PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.DepositorInsertSARS-CoV-2 Spike protein (D614G, N501Y) (S )
UseTagsExpressionMammalianMutationD614G, N501YPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT100
Plasmid#180512PurposePlasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
PlmCasX with DpbCasX R1 loop
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-5xT7-TtCsm
Plasmid#128572PurposeFor expression of T. thermophilus Csm in E. coli (Csm1-5)DepositorInsertsCsm1
Csm2, Csm3, Csm4, Csm5
UseTagsHRV 3C protease cleavage site and 10xHis tag on C…ExpressionBacterialMutationCodon optimized for expression in E. coli and Cod…PromoterT7 promoter, lac operatorAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAT527
Plasmid#180514PurposePlasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATZ-BFP-HA-GFP11
Plasmid#177721PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsGFP11, HA, and TagBFPExpressionBacterial and MammalianMutationE342K (ATZ mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only