168,238 results

1. pLVX-DmrB-Casp8-2A-mCherry-puro

   Plasmid

   #231971

   Purpose

   Tet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosis

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   Casp8 dimerizing construct with bi-cistronic mCherry (CASP8 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lck-mTurquoise2-iLID

   Plasmid

   #176125

   Purpose

   Plasma membrane localized iLID

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   iLID
   

   Expression

   Mammalian

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3_cAMPFIRE-L-R279E

   Plasmid

   #182282

   Purpose

   Mammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
   

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-iPASnFR-L

   Plasmid

   #225238

   Purpose

   Bacterial expression of iPASnFR-L

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+) K-Ras(WT)

   Plasmid

   #224283

   Purpose

   Cell transfection and expression of K-Ras

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   K-Ras (KRAS Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30a(+)-PE2

   Plasmid

   #180015

   Purpose

   Expressing PE2 protein in E.coli for protein purification

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   PE2
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Lac operator-controlled T7 promoter

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0656

   Plasmid

   #141653

   Purpose

   Lentiviral vector for overexpressing the CHD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CHD3 (CHD3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO-GAlpha13-RLuc8

   Plasmid

   #140986

   Purpose

   Encodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlpha13-RLuc8 (GNA13 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pATT-Dest

   Plasmid

   #79770

   Purpose

   Staging plasmid for use in DIP transposition

   Depositor

   David Savage

   Article

   Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

   Type

   Empty backbone

   Use

   Synthetic Biology; Staging plasmid for transposit…

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR-U6-gRNA-TRE3G-TdTomato

    Plasmid

    #192658

    Purpose

    A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Expression

    Mammalian

    Promoter

    U6, TRE3G

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB424

    Plasmid

    #74818

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD-iPASnFR-H

    Plasmid

    #225237

    Purpose

    Bacterial expression of iPASnFR-H

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRetroX-TRE3G_NT-GSDMD

    Plasmid

    #201170

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Mutation

    aa1-276 only

    Promoter

    TRE3G

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-Rhotekin(8-89)-mCherry-C1

    Plasmid

    #29675

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

    Insert

    Rhotekin (Rtkn Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 8-89

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. AbVec2.0-IGHG1

    Plasmid

    #80795

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-FLAG-LOV*-H2B

    Plasmid

    #202204

    Purpose

    Expresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Histone H2B type 1-K
    

    Tags

    FLAG and LOV*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHRE

    Plasmid

    #134909

    Purpose

    Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.

    Depositor

    George Lomonossoff

    Article

    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    double 35S

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSP1D1-Strep-II

    Plasmid

    #224209

    Purpose

    Membrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.

    Depositor

    Anne Robinson

    Article

    Pettersen et al Biophys J. 2025 Jan 21;124(2):440-457. d

    Insert

    MSP1D1-Strep-II
    

    Tags

    Strep-II Tag

    Expression

    Bacterial

    Mutation

    Substitution of 7xHis tag found in pMSP1D1 for a …

    Promoter

    T7

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-6P-acatenin

    Plasmid

    #186458

    Purpose

    Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

    Depositor

    Tina Izard

    Article

    Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBJ053_MTB_jGCaMP7f

    Plasmid

    #209280

    Purpose

    Ubiquitously expresses jGCaMP7f in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

    Depositor

    Adam Cohen

    Article

    Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

    Insert

    jGCaMP7f
    

    Use

    Zebrafish expression, in vitro transcription

    Promoter

    actb2

    Available Since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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