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pAAV-hSyn-DIO-mCherry (AAV8)

Viral Prep

#50459-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.

Depositor

Bryan Roth

Promoter

Syn

Tags

mCherry (Cre-dependent)

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-FLAG-hSF3B1-K700E

Plasmid

#82577

Purpose

Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-K700E (SF3B1 Human)

Tags

FLAG peptide

Expression

Mammalian

Mutation

K700E

Promoter

CMV

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_25bpLinker

Plasmid

#157788

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_25bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pICH47742::p35S-ePEmax3::tEURb7

Plasmid

#227692

Purpose

Golden Gate level 1, position 2, ePEmax3 expression cassette.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).

Use

CRISPR

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

Plasmid

#217971

Purpose

Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.

Depositor

Robert Piper

Article

Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

Insert

2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4

Expression

Mammalian

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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[#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient

Plasmid

#233506

Purpose

Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

DsRed loxp eGFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_sfCherry2(1-10)

Plasmid

#82602

Purpose

Expresses sfCherry2(1-10) fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(1-10)

Expression

Mammalian

Mutation

changed Glu 118 to Gln, changed Thr 128 to Ile

Promoter

CMV

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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All-in-one lenti-evoCDA-NG

Plasmid

#200449

Purpose

Lentivirus vector for base editing

Depositor

Benjamin Cravatt

Article

Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pIND20-KNL1-s24a_s60a-dCas9

Plasmid

#248563

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-s24a_s60a-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 S24A, S60A; Cas9 D10A, H840A

Promoter

TRE, miniCMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pN1-PinkyCaMP

Plasmid

#232861

Purpose

A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

Depositor

Olivia Masseck

Article

Fink et al Nat Methods. 2026 Apr 24. doi: 10.1038/s

Insert

PinkyCaMP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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hAAVS1 1L TALEN

Plasmid

#35431

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1L TALEN

Expression

Mammalian

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

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hAAVS1 1L TALEN

Plasmid

#35431

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1L TALEN

Expression

Mammalian

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

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pAJM.657

Plasmid

#108525

Purpose

CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

CymRAM + PCymRC-YFP

Expression

Bacterial

Mutation

CymR: S110G A171V

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET28HIS-hAPE1

Plasmid

#70757

Purpose

bacterial expression of human APE1

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

APEX1 (APEX1 Human)

Tags

6-HIS

Expression

Bacterial

Promoter

T7/lac operon

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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HDAC8 Flag

Plasmid

#13825

Depositor

Eric Verdin

Article

Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

Insert

HDAC8 (HDAC8 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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HDAC8 Flag

Plasmid

#13825

Depositor

Eric Verdin

Article

Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

Insert

HDAC8 (HDAC8 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pMYH9-WT-V5

Plasmid

#183512

Purpose

Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

Depositor

Emanuele Panza

Article

Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

Insert

MYH9 (MYH9 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF2340

Plasmid

#142039

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MITF (MITF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP670

Plasmid

#45466

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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