168,579 results

1. pcDNA_mK_Anti-HA [12CA5] v2

   Plasmid

   #236296

   Purpose

   Mammalian vector expressing Anti-HA [12CA5] variable region fused to mouse Kappa light chain; to be used with pcDNA_mIGg2a_Anti-HA [12CA5] v2 heavy chain (Plasmid 236295) to make the antibody.

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Anti-HA [12CA5] light chain variable region fused to mouse kappa light chain
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCAG mito-mTagBFP2

   Plasmid

   #105011

   Purpose

   Mitochondrial marker

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. DRD2-DuET

   Plasmid

   #213228

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   DRD2 (DRD2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hsyn-GRAB_Ado1.0mut

   Plasmid

   #153287

   Purpose

   Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in neurons

   Depositor

   Yulong Li

   Article

   Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

   Insert

   Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-Htt171-66Q-myc-WPRE

   Plasmid

   #107934

   Purpose

   Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

   Depositor

   Johan Jakobsson

   Article

   Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

   Insert

   Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)
   

   Use

   AAV

   Promoter

   hSynapsin

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GRM5-DuET

   Plasmid

   #213303

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GRM5 (GRM5 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVITRO-HPV11 L1L2

   Plasmid

   #52590

   Purpose

   Expresses HPV11 L1 and L2 in mammalian cells

   Depositor

   Richard Roden

   Article

   Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

   Inserts

   HPV11 L1
   HPV11 L2
   

   Expression

   Mammalian

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE

   Plasmid

   #106303

   Purpose

   The plasmid encodes the scFv against GCN4 peptide fused with HaloTag and a NLS for SunTag imaging

   Depositor

   James Zhe Liu

   Article

   Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

   Insert

   scFv-GCN4-HaloTag-GB1-NLS
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

   Plasmid

   #65458

   Purpose

   Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   tTA2
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV8)

    Viral Prep

    #112677-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

11. pJAT74

    Plasmid

    #204296

    Purpose

    pJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PABE-7

    Plasmid

    #115628

    Purpose

    Targeted A to G in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-3*NLS
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. DT0

    Plasmid

    #80418

    Purpose

    Expresses 0 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. vGAT-pH pcaggs SE

    Plasmid

    #78578

    Purpose

    image recycling of VGAT in live neurons

    Depositor

    Susan Voglmaier

    Article

    Santos et al J Neurosci. 2013 Jun 26;33(26):10634-46.

    Insert

    vGAT-pHluorin C-term (Slc32a1 Rat)
    

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Promoter

    chicken actin

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

15. Flag pLJM1 RagD wt

    Plasmid

    #19316

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding D (RRAGD Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEosEM-Lamin A

    Plasmid

    #132707

    Purpose

    application in Correlative light-electron microscopy

    Depositor

    Pingyong Xu

    Article

    Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    lamin A
    

    Tags

    mEosEM

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI-neo-RL-ZEB1

    Plasmid

    #35535

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    3'UTR of ZEB1 (ZEB1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_TRAF3_WT

    Plasmid

    #81755

    Purpose

    Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TRAF3 (TRAF3 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-dCas9-mD3A

    Plasmid

    #78257

    Purpose

    Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoter

    Depositor

    Grant Challen

    Article

    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

    Insert

    DNMT3A CD aa 598-912 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    E756A

    Promoter

    pCMV

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC020 - LseCas13a from Listeria seeligeri

    Plasmid

    #91910

    Purpose

    Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LseCas13a and guide expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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