171,944 results
-
Plasmid#49343PurposepHAGE NFkB-TA-LUC-UBC-GFP-WDepositorInsertsUseLentiviralAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only
-
mito NAD+ biosensor
Plasmid#186791PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondriaDepositorInsertmito NAD+ biosensor
UseLentiviralExpressionMammalianAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64
Plasmid#203348PurposeTemplate for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertEnhanced Green Fluorescent Protein
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …PromoterT7Available SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcs2-GFP-IRES-mCherry
Plasmid#226098PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCAS9-VP64_GFP
Plasmid#61422PurposeExpresses dCAS9-VP64 activator with 2A GFPDepositorHas ServiceConcentrated Lentiviral PrepInsertdCAS9(D10A,H840A)-VP64_2A_GFP
UseCRISPR and LentiviralExpressionMammalianMutationD10A and H840A in Cas9PromoterEF1AAvailable SinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX307 P2A GFP Luciferase
Plasmid#193674PurposeConstitutive lentiviral expression of LuciferaseDepositorInsertLuciferase
UseLentiviralAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EGFP-3XNLS
Plasmid#58468PurposeExpresses a nuclear-targeted eGFP on a CMV promoterDepositorInsert3X NLS with 3X stop codons
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-eGFP-Puro
Plasmid#79823PurposeExpresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.DepositorAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cFos-tTA-pA
Plasmid#66794PurposeExpresses TetActivator protein under the control of the c-fos promoter for use in TetTagingDepositorInsertcfos promoter linked to the tet activator gene
UseAAVTagsnoExpressionMammalianAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-iGluSnFR.A184S (AAV1)
Viral Prep#106174-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.hSynapsin.SF-iGluSnFR.A184S (#106174). In addition to the viral particles, you will also receive purified pAAV.hSynapsin.SF-iGluSnFR.A184S plasmid DNA. hSynapsin driven SF-iGluSnFR.A184S, glutamate sensor. The A184S mutant has a higher glutamate affinity. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8s-WPRE (AAV9)
Viral Prep#162374-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL-3F-BirA
Plasmid#220147PurposeExpresses VHL-BirA for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationcodon optimizedPromoterEF-1aAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXPR_050
Plasmid#96925Purposelentiviral expression of gRNA scaffoldDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
IKK-2 S177E S181E
Plasmid#11105PurposeMammalian expression of constitutively active human IKK-2DepositorInsertIKK-2 (IKBKB Human)
TagsFLAGExpressionMammalianMutationS177E and S181E, constitutively activeAvailable SinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSPgRNA
Plasmid#47108PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cellsDepositorHas ServiceCloning Grade DNAInsertSPgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ATR Kinase dead
Plasmid#31612DepositorAvailable SinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-RFP
Plasmid#1817PurposeMammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
TagsRFPExpressionMammalianMutationD50E (not important for function of plasmid)Available SinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRE-dCas9-KRAB-MeCP2-BSD
Plasmid#140690PurposeInducible knockdown of gene expression in human cells for pooled scRNA-seq experimentsDepositorInsertCas9m4-KRAB-MeCP2
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Bidirectional MYC 5'UTR Dual Fluorescence Reporter
Plasmid#229498PurposeThis is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.DepositorInsertMYC 5'Untranslated region + destabilized GFP
UseLentiviralExpressionMammalianPromoterEf1aAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
VR122 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198467PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
Tags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only