264,503 results

1. pCAG-Kir2.1Mut-T2A-tdTomato

   Plasmid

   #60644

   Purpose

   Expresses Kir2.1Mut-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   Kcnj2 (Kcnj2 Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GYG to AAA (aa144-146)

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Kir2.1Mut-T2A-tdTomato

   Plasmid

   #60644

   Purpose

   Expresses Kir2.1Mut-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   Kcnj2 (Kcnj2 Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GYG to AAA (aa144-146)

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCry2PHR-mCh-LRP6c

   Plasmid

   #42960

   Purpose

   Expresses optogenetically controlled LRP6 (C-terminal domain)

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-LRP6c
   

   Expression

   Mammalian

   Mutation

   LPR6 cytoplasmic domain only

   Promoter

   CMV

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCry2PHR-mCh-LRP6c

   Plasmid

   #42960

   Purpose

   Expresses optogenetically controlled LRP6 (C-terminal domain)

   Depositor

   David Schaffer

   Article

   Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

   Insert

   Cry2PHR-mCherry-LRP6c
   

   Expression

   Mammalian

   Mutation

   LPR6 cytoplasmic domain only

   Promoter

   CMV

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. mu-FLT3Lv1-pTRIP

   Plasmid

   #163519

   Purpose

   Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

   Depositor

   Thomas Weber

   Insert

   murine-FLT3L variant 1 (Flt3l Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-hEAAT2

   Plasmid

   #32814

   Depositor

   Susan Amara

   Insert

   hEAAT2 (SLC1A2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-hEAAT2

   Plasmid

   #32814

   Depositor

   Susan Amara

   Insert

   hEAAT2 (SLC1A2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCAR_sgRNA_hygro-tagBFP-lox2272

   Plasmid

   #162078

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPRyl_AXP

   Plasmid

   #84608

   Purpose

   Introduces DSB at AXP locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPRyl_AXP

    Plasmid

    #84608

    Purpose

    Introduces DSB at AXP locus in Y. lipolytica

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CMV-MCS-Puro

    Plasmid

    #219794

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBADTrfp

    Plasmid

    #99382

    Purpose

    This promoter system provided the highest RFP expression upon induction

    Depositor

    Nathan Hillson

    Insert

    RFP
    

    Expression

    Bacterial

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBADTrfp

    Plasmid

    #99382

    Purpose

    This promoter system provided the highest RFP expression upon induction

    Depositor

    Nathan Hillson

    Insert

    RFP
    

    Expression

    Bacterial

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR_D17_hrGFP

    Plasmid

    #84616

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_D17_hrGFP

    Plasmid

    #84616

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    UAS1B8-TEF(136)

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-FGFR1

    Plasmid

    #23922

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FGFR1 (FGFR1 Human)
    

    Use

    Gateway donor vector

    Mutation

    W211G and S351F compared to insert sequence provi…

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223-FGFR1

    Plasmid

    #23922

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FGFR1 (FGFR1 Human)
    

    Use

    Gateway donor vector

    Mutation

    W211G and S351F compared to insert sequence provi…

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-eNme2-C-BE4max

    Plasmid

    #183679

    Purpose

    Expresses eNme2-C-BE4 in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    rAPOBEC1-neNme2-C-2xUGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Anti-TrpC4 [N77/15.1R]

    Plasmid

    #114504

    Purpose

    Mammalian Expression Plasmid of anti-TrpC4 (Rat). Derived from hybridoma N77/15.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TrpC4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpc4 Mouse)
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Anti-TrpC4 [N77/15.1R]

    Plasmid

    #114504

    Purpose

    Mammalian Expression Plasmid of anti-TrpC4 (Rat). Derived from hybridoma N77/15.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TrpC4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpc4 Mouse)
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge