172,556 results

Showing: 1521 - 1540 of 172556 results

Results per page:

Sort results by:

pDONR221-SLC39A7_STOP

Plasmid

#161331

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A7 (SLC39A7 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Blast-3xHA

Plasmid

#207685

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
TFORF3084

Plasmid

#144560

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZFP36L1 (ZFP36L1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-p62-TIR-AAA

Plasmid

#187901

Purpose

Mammalian cell expression of a triple mutant of p62: P228A, E230A, P232A

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

SQSTM1 (SQSTM1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Triple alanine mutant: P228A, E230A, P232A

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GRM4-Tango

Plasmid

#66389

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GRM4 (GRM4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pSLS.491

Plasmid

#184956

Purpose

Test effect of a1/a2 length on editing rpoB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12

Expression

Bacterial

Mutation

rpoB donor T1534C

Promoter

T7/lac

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLS.436

Plasmid

#184953

Purpose

Express -Eco1 RT and ncRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: ncRNA(wt) and RT

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRF TpC

Plasmid

#171039

Purpose

Empty vector for Firefly-Renilla luciferase assay for hTERT promoter activity

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL2620 (pTniQ_Vch)

Plasmid

#200892

Purpose

Expresses human codon-optimized V. cholerae TniQ from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchTniQ

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNP561

Plasmid

#222723

Purpose

Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Tags

6xHis

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBI-eGFP-Rab31

Plasmid

#200265

Purpose

Expression of eGFP-Rab31 (human) in mammalian cells

Depositor

Guangpu Li

Article

Banworth et al J Biol Chem. 2022 Sep;298(9):102281. doi

Insert

Rab31 (human cDNA) (RAB31 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgLkb1/Cre

Plasmid

#66894

Purpose

Expresses an Lkb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

Insert

sgLkb1

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-N-Zeo-V5

Plasmid

#207708

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-V5 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
3XFlag DVL2 (∂DIX)

Plasmid

#24805

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of the N-terminal DIX domain

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
FLAG HDAC5 S259A/S498A

Plasmid

#32216

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser259Ala, Ser498Ala

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a

Plasmid

#58380

Purpose

tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

avi-EGFP-rpl10a

Tags

Avi Tag and EGFP

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
SUMO_TwinStrep_Csx30

Plasmid

#197611

Purpose

For protein purification of Csx30

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

SUMO_TwinStrep_Csx30

Expression

Bacterial

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL2621 (pCas8_Vch)

Plasmid

#200893

Purpose

Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas8

Tags

NLS

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL2623 (pCas6_Vch)

Plasmid

#200895

Purpose

Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas6

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mPlum-VE-Cadherin-N-10

Plasmid

#56005

Purpose

Localization: Tight Junctions, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

VE-Cadherin (CDH5 Human)

Tags

mPlum

Expression

Mammalian

Mutation

I503T

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge