169,069 results

1. pEN019-SpCas9-hiNLS-t-M1M4

   Plasmid

   #234456

   Purpose

   Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

   Depositor

   Ross Wilson

   Article

   Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

   Insert

   SpCas9 hiNLS t-M1M4
   

   Use

   CRISPR

   Tags

   His6, Nucleoplasmin, SV40, and c-Myc

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV2-miniSOG-VAMP2-T2A-mCherry

   Plasmid

   #50970

   Purpose

   AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct

   Depositor

   Roger Tsien

   Article

   Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

   Insert

   miniSOG-VAMP2-T2A-mCherry (Vamp2 Arabdopsis thaliana, Mouse)
   

   Use

   AAV

   Tags

   T2A-mCherry and miniSOG

   Promoter

   human synapsin promoter

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF2307

   Plasmid

   #142023

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ETV1 (ETV1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-SLC22A6_STOP

   Plasmid

   #161131

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC22A6 (SLC22A6 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAAV-CaMKII-Ace-mNeon2-Kv2.1PR

   Plasmid

   #195528

   Purpose

   Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   Ace-mNeon2-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Ace-mNeon 81S; SY linker

   Promoter

   CaMKII

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNSEN-d2

   Plasmid

   #59763

   Purpose

   Expresses nuclear EGFP and Tomato in mammalian cells

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi

   Inserts

   d2-EGFP
   Tomato
   

   Tags

   3X NLS and PEST

   Expression

   Mammalian

   Promoter

   CMV promoter and SV40 promoter

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPBCAG-rtTAM2-IH

   Plasmid

   #140827

   Purpose

   Expresses rtTAM2-IRES-HygR in mammalian cells

   Depositor

   Hideyuki Okano

   Article

   Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

   Inserts

   rtTAM2
   HygR
   

   Expression

   Mammalian

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Dlx-FLEX-hM4Di-mCherry

   Plasmid

   #209701

   Purpose

   Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

   Depositor

   Peer Wulff

   Article

   Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

   Insert

   hM4D(Gi)
   

   Use

   AAV

   Tags

   mCherry

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV-AR-KKKAAA

   Plasmid

   #171219

   Purpose

   Lenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.

   Depositor

   Steven Balk

   Article

   Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

   Insert

   AR-KKKAAA (AR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-K630A-K632A-K633A

   Promoter

   EF-1a

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET22b-ecDHFR

    Plasmid

    #109055

    Purpose

    Bacterial expression of E.coli DHFR protein

    Depositor

    Stephen Benkovic

    Article

    Cameron et al Biochemistry. 1997 Dec 16;36(50):15792-8

    Insert

    ecDHFR
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-Synapsin-mCherry-3XNLS-WPRE

    Plasmid

    #159951

    Purpose

    nucleus marker

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mCherry-3XNLS
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221_SLCO2B1

    Plasmid

    #131866

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLCO2B1 (SLCO2B1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. mCD4 e/p Sal-(hCD2)

    Plasmid

    #24070

    Depositor

    Dan Littman

    Article

    Sawada et al Cell. 1994 Jun 17. 77(6):917-29.

    Insert

    CD2 (CD2 Human)
    

    Tags

    mCD 4 promoter

    Expression

    Mammalian

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

14. ADORA1-DuET

    Plasmid

    #213173

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADORA1 (ADORA1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1-puro-irf3-shrna5

    Plasmid

    #127649

    Purpose

    Knock-down of human IRF3

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    IRF3 shRNA (IRF3 Human)
    

    Use

    Lentiviral

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. plex_307-3xflag-ccdb-blasticidin

    Plasmid

    #201123

    Purpose

    pLEX_307 plasmid with N-terminal FLAG tag and blasticidin marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV-AR-C563A-C596A

    Plasmid

    #171220

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-C563A-C596A (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-C563A-C596A

    Promoter

    EF-1a

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV-AR-C619Y

    Plasmid

    #171222

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-C619Y (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-C619Y

    Promoter

    EF-1a

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221_SLC22A8

    Plasmid

    #131989

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC22A8 (SLC22A8 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pT3-Neo

    Plasmid

    #69123

    Purpose

    Sleeping Beauty (SB) transposon with G418 resistance

    Depositor

    Martin Bonamino

    Article

    Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

    Insert

    Neo (codon optimized for human)
    

    Use

    Sleeping beauty transposon

    Expression

    Mammalian

    Promoter

    U3MSCV

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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