168,756 results

1. pLVX-puro-cOVA-IRES-BFP

   Plasmid

   #135074

   Purpose

   Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.

   Depositor

   Maria Castro

   Insert

   cOVA-IRES-BFP (OVAL Chicken)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEVOL-pAzF

   Plasmid

   #31186

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

   Depositor

   Peter Schultz

   Article

   Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

   Insert

   M.j. p-azidohenylalanine RS (2 copies +tRNA)
   

   Expression

   Bacterial

   Mutation

   Y32T, E107N, D158P, I159L, L162Q, D286R

   Available Since

   Dec. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRY2olig-mCherry

   Plasmid

   #60032

   Purpose

   Expresses a fusion of CRY2PHR E490G with mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2PHR E490G (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   E490G

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5_mEGFP-NUP98-HOXA9

   Plasmid

   #237639

   Purpose

   For overexpression of mEGFP-NUP98-HOXA9

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   NUP98-HOXA9 (NUP98, HOXA9 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRH-1346 U6-B2M sgRNA Gag-pol v2

   Plasmid

   #201917

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-pol
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Expression

   Mammalian

   Promoter

   CAG and Human U6

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9

   Plasmid

   #167547

   Purpose

   Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Inserts

   chloramphenicol acetyl transferase
   pUC19 origin of replication
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-CMV-C-EGFP

   Plasmid

   #122844

   Purpose

   C-terminal EGFP tag. Gateway destination vector for mammalian expression.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HDM-Hgpm2

   Plasmid

   #204152

   Purpose

   Lentivirus helper plasmid coding for Gag/Pol

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   HIV-1 gag/pol
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. HDM-tat1b

   Plasmid

   #204154

   Purpose

   Lentivirus helper plasmid coding for Tat

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   tat
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. H2B-GFP

    Plasmid

    #11680

    Depositor

    Geoff Wahl

    Article

    Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

    Insert

    Human histone H2B (H2BC11 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRC-CMV-Rev1b

    Plasmid

    #204153

    Purpose

    Lentivirus helper plasmid coding for Rev

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    Rev
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-syn-iGluSnFR4f-NGR-WPRE (AAV1)

    Viral Prep

    #234438-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-syn-iGluSnFR4f-NGR-WPRE (#234438). In addition to the viral particles, you will also receive purified pAAV-syn-iGluSnFR4f-NGR-WPRE plasmid DNA. Syn-driven, iGluSnFR4f-NGR sensor expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Promoter

    Syn

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

13. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-CMV-N-EGFP

    Plasmid

    #122842

    Purpose

    N-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-GRAB_ACh3.0

    Plasmid

    #121922

    Purpose

    express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

    Depositor

    Yulong Li

    Article

    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

    Has Service

    AAV9

    Insert

    GPCR activation based ACh sensor GRAB-ACh3.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT4-CMV-GFP

    Plasmid

    #117046

    Purpose

    Optimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoter

    Depositor

    Wolfgang Uckert

    Insert

    GFP
    

    Use

    Transposon

    Promoter

    CMV

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJRH-1328 LV EF1a-CD20 IRES-EGFP

    Plasmid

    #201918

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD20
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. HDM_RSV_B1_G_31AACTdel

    Plasmid

    #237354

    Purpose

    Expression plasmid for RSV B1 G

    Depositor

    Jesse Bloom

    Article

    Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

    Insert

    RSV B1 G human codon optimized from Genbank Accesion AF013254
    

    Expression

    Mammalian

    Mutation

    31 amino acid cytoplasmic tail deletion

    Promoter

    CMV

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-GFP

    Plasmid

    #37825

    Purpose

    AAV-mediated expression of GFP under the CAG promoter

    Depositor

    Edward Boyden

    Has Service

    AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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