168,428 results

1. pFastBac1-Ac-AChBP

   Plasmid

   #63128

   Purpose

   Expression of Aplysia californica AChBP

   Depositor

   Titia Sixma

   Article

   Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

   Insert

   AChBP
   

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCL52.2

   Plasmid

   #13656

   Depositor

   Chia Lee

   Article

   Sau et al J Bacteriol. 1997 Mar . 179(5):1614-21.

   Type

   Empty backbone

   Use

   E. coli-s. arueus shuttle vector

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA

   Plasmid

   #156439

   Purpose

   For transient expression of mouse PDS5A tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse PDS5A (Pds5a Mouse)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFBOH-LIC_DDB1:1-1140_delta:396-689

   Plasmid

   #210897

   Purpose

   Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
   

   Expression

   Insect

   Mutation

   loop deletion amino acids I396 to D689

   Promoter

   Polyhedrin

   Available Since

   March 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLP-mScarlet-I

   Plasmid

   #209993

   Purpose

   Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   mScarlet-I
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PRKDC gRNA (BRDN0001149021)

   Plasmid

   #77864

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKDC

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKDC (PRKDC Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-2xFLAG-2xSTREP-CDK2

   Plasmid

   #172616

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK2 (CDK2 Human)
   

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pD649-HAsp-KIR3DS1-COMP5AP-AviTag-9xHis

   Plasmid

   #157323

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR3DS1 (KIR3DS1 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM60-FLAG NUFIP1

    Plasmid

    #110950

    Purpose

    expresses mammalian FLAG-NUFIP1

    Depositor

    David Sabatini

    Article

    Wyant et al Science. 2018 May 18;360(6390):751-758.

    Insert

    NUFIP1 (NUFIP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-32a(+)-TGFB3m

    Plasmid

    #120289

    Purpose

    Produce human recombinant mTGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    monomeric Transforming Growth Factor-β3
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-BBS1

    Plasmid

    #218711

    Purpose

    Expresses N-terminally EGFP-tagged BBS1 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    BBS1 (BBS1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO-mito-mCer

    Plasmid

    #185602

    Purpose

    Lentiviral construct for expression of mCerulean targeted to mitochondria

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    mito-mCer
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDblet

    Plasmid

    #8848

    Depositor

    Joel Huberman

    Article

    Brun et al Gene. 1995 Oct 16. 164(1):173-7.

    Insert

    ARS doublet
    

    Use

    Yeast cloning vector

    Expression

    Yeast

    Mutation

    This vector contains, between AatII sites, a dire…

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCLR2070

    Plasmid

    #28063

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains N496D point mutation in FokI domain, as …

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCLR2068

    Plasmid

    #28062

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains H537R point mutation in FokI domain, as …

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. His-DNPH1

    Plasmid

    #203224

    Purpose

    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    DNPH1 (DNPH1 Human)
    

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sticky-355-3X

    Plasmid

    #159644

    Purpose

    Non-coding DNA sequence for dinucleosome preparation

    Depositor

    Curt Davey

    Article

    Adhireksan et al Nat Commun. 2020 Sep 21;11(1):4747. doi:

    Type

    Empty backbone

    Use

    Bacterial vector for dna production

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSP64T acvr1ba*

    Plasmid

    #185409

    Purpose

    Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    acvr1ba (acvr1ba Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Expression

    Mammalian

    Mutation

    changed Threonine 206 to Aspartic acid

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGY416

    Plasmid

    #166660

    Purpose

    For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox

    Expression

    Yeast

    Mutation

    Cre: E340A, D341A

    Promoter

    GAL1

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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