169,057 results

1. pEG BacMam C term GFP His8

   Plasmid

   #160681

   Purpose

   Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Tags

   His8 GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS U6a gRNA

   Plasmid

   #158961

   Purpose

   gRNA backbone driven by zebrafish U6a promoter

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   zf U6a promoter
   gRNA backbone
   

   Use

   CRISPR

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. ssFv_lenti_wtATMSPARK2

   Plasmid

   #195249

   Purpose

   express wtATMSPARK2 in a lenti vector

   Depositor

   Xiaokun Shu

   Article

   Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

   Insert

   ATMSPARK2 sensor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   ssfv

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57-RFP-P2A-MCS (Neo)

   Plasmid

   #89182

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Human RNF149-3X FLAG

   Plasmid

   #225332

   Purpose

   Lentiviral expression of human RNF149 with 3x FLAG tag in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   RNF149 (RNF149 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGPD2

   Plasmid

   #43972

   Depositor

   Hal Alper

   Article

   Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Promoter

   GPD

   Available Since

   April 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. ABEmax-Puro V3

   Plasmid

   #226956

   Purpose

   ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUAP1

   Plasmid

   #63674

   Purpose

   Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.

   Depositor

   Nicola Patron

   Article

   Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np

   Insert

   RFP cloning selection cassette
   

   Use

   Synthetic Biology

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-enAsCas12f

   Plasmid

   #204637

   Purpose

   Expression of enAsCas12f in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   enAsCas12f
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D196K, N199K, G276R, N328G, D364R

   Promoter

   CMV

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 8138-E02

    Plasmid

    #225659

    Purpose

    Gateway clone for SORE6 reporter (6 repeats of a SOX2/OCT4 response element from the human NANOG promoter)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    SORE6
    

    Use

    Gateway entry clone

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-NUP188

    Plasmid

    #159893

    Purpose

    Expresses NUP188 in mammalian cells

    Depositor

    Kozo Tanaka

    Article

    Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

    Insert

    NUP188 (NUP188 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    To avoid NotI site, first three amino acids are d…

    Promoter

    CMV

    Available Since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJMP3664

    Plasmid

    #222350

    Purpose

    Expression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

    Depositor

    Jason Peters

    Article

    Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ntn1-Fc-His

    Plasmid

    #72104

    Purpose

    Expresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn1 (Ntn1 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. WT WWP1

    Plasmid

    #200085

    Purpose

    WWP1 in pQCXIN vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    WT WWP1 (WWP1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-Lin28B

    Plasmid

    #51373

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    Lin28B (LIN28B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pELPS-eDHFR-YFP-T2A-Renilla

    Plasmid

    #193253

    Purpose

    Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase

    Depositor

    Mark Sellmyer

    Article

    Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1

    Insert

    The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    

    Use

    Lentiviral

    Tags

    Renilla luciferase (rLuc) and yellow fluorescent …

    Mutation

    None

    Promoter

    EF1a

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMALcPP-MS2cp

    Plasmid

    #203351

    Purpose

    Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

    Depositor

    Thomas Cech

    Article

    Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

    Insert

    MS2 coat protein
    

    Tags

    Hx6-MBP

    Expression

    Bacterial

    Mutation

    V75E and A81G, to prevent capsid assembly

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid

    #89180

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKIKOarsBCm

    Plasmid

    #46763

    Purpose

    E.coli genomic integration at arsB

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

    Type

    Empty backbone

    Use

    E.coli genomic integration

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV GFAP CaBLAM

    Plasmid

    #244228

    Purpose

    Bioluminescent reporter for calcium signaling in astrocytes

    Depositor

    Ute Hochgeschwender

    Article

    Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

    Inserts

    mNeonGreen
    CaBLAM
    

    Use

    AAV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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