173,139 results
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Plasmid Kit#1000000171PurposeMoClo-compatible vectors for assembly of CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants.DepositorApplicationCloning and Synthetic Biology, Genome EditingVector TypePlant ExpressionEditing TypeCRISPRCloning TypeGolden Gate (MoClo)Available SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only
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hSyn-DIO-somBiPOLES-mCerulean (AAV5)
Viral Prep#154951-AAV5PurposeReady-to-use AAV5 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCerulean (Cre-dependent)Available SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX_311-KRAB-dCas9
Plasmid#96918Purposefor CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121DepositorInsertdCas9
UseLentiviralTagsKRABExpressionMammalianMutationdCas9PromoterEF1aAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII 0.4.Cre.SV40 (AAV1)
Viral Prep#105558-AAV1PurposeReady-to-use AAV1 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIIAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato (AAV9)
Viral Prep#107738-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLX1.8NFATGFPpd2HS4
Plasmid#169096PurposeLentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activationDepositorInsertsNFAT enhancer (from IL-4 promoter)
Destabilized green fluorescent protein
UseLentiviralPromoterNFAT enhancer + synthetic TATA boxAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE57
Plasmid#90402PurposeType I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterISREAvailable SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon-ChRmine-oScarlet (AAV8)
Viral Prep#137159-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-ChRmine-oScarlet (#137159). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-ChRmine-oScarlet plasmid DNA. nEF-driven, Cre and Flp-dependent expression of ChRmine-oScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsoScarlet (Cre and Flp-dependent)Available SinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNeg-Ma-barnase-TAG3-TAG45
Plasmid#197572PurposeNegative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertsBarnase - 2xTAG
M. alvus Pyl-tRNA (6)
TagsnoneExpressionBacterialMutation3TAG and 45TAGPromoteraraC and lppAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Blast
Plasmid#111887PurposeLentiviral expression of cDNA with blasticidin resistance geneDepositorTypeEmpty backboneUseLentiviralTagsFLAGExpressionMammalianPromoterEFS promoterAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV1)
Viral Prep#28306-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-(-)-Pur
Plasmid#200458PurposeEukaryotic expression vectorDepositorTypeEmpty backboneExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)
Plasmid#149276PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorInsertModified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
UseT-dna vectorMutationT2318C (DNA) in PEBV coat protein sequenceAvailable SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter
Plasmid#61762PurposeDual Color betaGlobin reporter to study splicing kinetics in vivoDepositorInsertb-Globin
Tags24 X MS2 hairpin cassette in 3'UTR of b-Glob…ExpressionMammalianPromoterDox inducible Tet-ON promoterAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV G-CEPIA1er
Plasmid#58215PurposeGreen fluorescent indicator for calcium imaging in the endoplasmic reticulumDepositorInsertG-CEPIA1er
TagsmycExpressionMammalianMutationG-GECO1.1 E299D M304L D328N N345K F360W G369S E37…PromoterCMVAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.S
Plasmid#103006Purposenon-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.S VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Sec61b-C1
Plasmid#90992PurposeExpresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cellsDepositorInsertmEmerald-Sec61b (SEC61B Synthetic, Human)
TagsmEmerald (green fluorescence)ExpressionMammalianPromoterCMVAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64
Plasmid#203348PurposeTemplate for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertEnhanced Green Fluorescent Protein
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …PromoterT7Available SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TFEB-WT-MYC (#647)
Plasmid#99955Purposemammalian expressionDepositorAvailable SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
PP7_mCherry
Plasmid#61763PurposeExpresses PP7 coat protein fused to mCherryDepositorInsertPP7 coat protein fused to mCherry
UseLentiviralTagsmCherryExpressionMammalianPromoterUbc promoterAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only