200,493 results

1. pAAV-flex-taCasp3-TEVp (AAV1)

   Viral Prep

   #45580-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-flex-taCasp3-TEVp (#45580). In addition to the viral particles, you will also receive purified pAAV-flex-taCasp3-TEVp plasmid DNA. EF1a-driven, Cre-dependent, bicicstronic expression of designer pro-taCasp3 and TEVp. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Nirao Shah, Jim Wells

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Promoter

   EF1a

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3 RLUC POLIRES FLUC

   Plasmid

   #45642

   Purpose

   Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

   Depositor

   Nahum Sonenberg

   Article

   Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

   Insert

   RLUC - IRES - FLUC
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 RLUC POLIRES FLUC

   Plasmid

   #45642

   Purpose

   Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

   Depositor

   Nahum Sonenberg

   Article

   Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

   Insert

   RLUC - IRES - FLUC
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1

   Plasmid

   #41393

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1

   Plasmid

   #41393

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV5)

   Viral Prep

   #44361-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Promoter

   Syn

   Tags

   mCherry (Cre-dependent)

   Available Since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

7. MoClo Plant Parts III: Transformation & Genome Engineering Kit

   Plasmid Kit

   #1000000236

   Purpose

   The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.

   Depositor

   Daniel Voytas

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV9)

   Viral Prep

   #20298-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   EF1a

   Tags

   EYFP (Cre-dependent)

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pBBR1MCS-2

   Plasmid

   #85168

   Purpose

   Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

   Depositor

   Kenneth Peterson

   Article

   Kovach et al Gene. 1995 Dec 1;166(1):175-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Constitutive (derepressed P-lac)

   Available Since

   Nov. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAAV.Syn.GCaMP6f.WPRE.SV40 (AAV9)

    Viral Prep

    #100837-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.Syn.GCaMP6f.WPRE.SV40 (#100837). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-Syn-ChrimsonR-tdT (AAV5)

    Viral Prep

    #59171-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    Syn

    Tags

    tdTomato

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pMIG-FLAG-MLL-AF9

    Plasmid

    #71443

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-Af9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. MLM3636

    Plasmid

    #43860

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

    Depositor

    Keith Joung

    Insert

    None
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. MLM3636

    Plasmid

    #43860

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter

    Depositor

    Keith Joung

    Insert

    None
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-M-puro

    Plasmid

    #125839

    Purpose

    modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TRE-dCas9-VPR

    Plasmid

    #63800

    Purpose

    SP-dCas9-VPR with doxycycline-inducible expression

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSETB/mStayGold

    Plasmid

    #212017

    Purpose

    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-Bsd-CMV>tTS/rtTA:T2A:{cymR}

    Plasmid

    #236251

    Purpose

    Blasticidin selected lentiviral vector with Tet-On regulatory proteins and the cumate repressor, for use with dual-inducible CymR-CuO system

    Depositor

    Genevieve Kendall

    Article

    Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

    Inserts

    tTS/rtTA
    cymR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    rTS and rtTA_M2 linked by T2A

    Promoter

    CMV

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPLICS Mt-ER Short P2A

    Plasmid

    #164108

    Purpose

    Detect the short Mitochondria-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Mt-ER Short P2A
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. S2060

    Bacterial Strain

    #105064

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

    Bacterial Resistance

    Streptomycin and tetracycline

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only