Addgene

We narrowed to 24,565 results for: sta

Showing: 1501 - 1520 of 24565 results

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pCold I-Hero9

Plasmid

#187927

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero9

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero9

Use

Tags

Flag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pVk7-33G1-Flag

Plasmid

#58950

Purpose

backbone for mouse recombinant antibody with added C-terminal FLAG tag

Depositor

Gavin Wright

Article

Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

Type

Empty backbone

Use

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pJL1-aadA

Plasmid

#117050

Purpose

In vitro expression of aadA (streptomycin resistance gene) from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

aadA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-AUA-IRES-mCherry

Plasmid

#222116

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AUA start codon

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AGC-IRES-mCherry

Plasmid

#222119

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AGC start codon

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-CUG-IRES-mCherry

Plasmid

#222110

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

CUG start codon

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-ACG-IRES-mCherry

Plasmid

#222113

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

ACG start codon

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-UUG-IRES-mCherry

Plasmid

#222112

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

UUG start codon

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AUU-IRES-mCherry

Plasmid

#222118

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AUU start codon

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AGG-IRES-mCherry

Plasmid

#222115

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AGG start codon

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AUC-IRES-mCherry

Plasmid

#222117

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AUC start codon

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-FRB-Rac1/N12

Plasmid

#214294

Purpose

Express split-Rac1 fragment fused with FRB and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-Rac1 fragment fused with FRB (RAC1 Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Promoter

Tac

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b-Rac1/13C-FKBP-MBP

Plasmid

#214295

Purpose

Express split-Rac1 fragment fused with FKBP and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-Rac1 fragment fused with FKBP (RAC1 Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Rac1-Q61L

Promoter

T7

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_TFP_WPRE_polyA

Plasmid

#220223

Purpose

AAV-STARR-seq validation control vector with TFP

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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185_pETcon_SARS2_BQ1-1

Plasmid

#208085

Purpose

yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 BQ.1.1 RBD (S Budding Yeast, SARS-CoV-2 virus)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-neoR

Plasmid

#117048

Purpose

In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

neoR

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJK347

Plasmid

#71833

Purpose

Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genes

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Inserts

hypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Use

Tags

Expression

Bacterial

Mutation

changes histidine-59 to aspartate

Promoter

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK348

Plasmid

#71832

Purpose

Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genes

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Inserts

hypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Use

Tags

Expression

Bacterial

Mutation

changes histidine-59 to asparagine

Promoter

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSG4K5

Plasmid

#74492

Purpose

For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

Depositor

Xiao Wang

Article

Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-mCherry

Plasmid

#75161

Purpose

Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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