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Plasmid#137722PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)DepositorInsertBRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTruncated at amino acid 1328PromoterTight TRE promoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso CdelET
Plasmid#137723PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)DepositorInsertBRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationDeleted amino acids 600-682PromoterTight TRE promoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-NMYC-hTRF1deltaBLM
Plasmid#64165PurposeRetroviral vector expressing human TRF1 delta aa317-374 with N-terminal MYC tagDepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsMycExpressionMammalianMutationdeleted amino acids aa317-374 of human TRF1PromoterCMVAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEPT_Cdk2-T160E-siR
Plasmid#73976PurposeHDR template to generate Cdk2-T160E-siRDepositorUseAAVTagsExpressionMutationT160 mutated to Glu and siRNA target site mutatedPromoterAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW-ND-Cdt1Δ499-546-HA-Puro
Plasmid#193768PurposeDoxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertND-Cdt1Δ499-546 (CDT1 Human)
UseLentiviralTagsHAExpressionMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-WRN K577M
Plasmid#125790Purposeconstitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)DepositorInsertmyc-WRN K577M (WRN Human)
UseTags5x mycExpressionMammalianMutationK577M (helicase-dead)PromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-WRN E84A K577M
Plasmid#125791Purposeconstitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRNDepositorInsertmyc-WRN E84A/K577M (WRN Human)
UseTags5x mycExpressionMammalianMutationE84A/K577M (exonuclease/helicase-dead)PromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast
Plasmid#193139PurposeExpresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistanceDepositorUseLentiviralTagsExpressionMutationhuman Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…PromoterEF1aAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OKS-iG
Plasmid#58974PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OKS-iM
Plasmid#58972PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoter and IRESAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-OKS-iM
Plasmid#58973PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-OKS-iG
Plasmid#58975PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Dynein-2_complex
Plasmid#132536PurposeFull length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.DepositorInsertsUseTags8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …ExpressionInsectMutationCodon optimised for Sf9 expressionPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS12-mCherry
Plasmid#25444DepositorInsertmCherry
UseTagsCOX4 PresequenceExpressionYeastMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pmEYFP-1
Plasmid#38773DepositorTypeEmpty backboneUseTagsmEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmECFP-1
Plasmid#38772DepositorTypeEmpty backboneUseTagsmECFPExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204TKC-DsRed-Express2-HDEL
Plasmid#21770DepositorInsertDsRed-Express2
UseTagsHDEL and preKar2ExpressionYeastMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDG03769
Plasmid#195996PurposeNanobody Proj-Nano28 productionDepositorInsertH14-NEDD8- ProjNano28-Cys
UseE.coli expressionTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDG04135
Plasmid#196013PurposeNanobody Zasp-Nano65 productionDepositorInsertH14-SUMO-Cys- ZaspNano65-Cys
UseE.coli expressionTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDG03781
Plasmid#195995PurposeNanobody Sls-Nano48 productionDepositorInsertH14-NEDD8- SlsNano48-Cys
UseE.coli expressionTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only