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Plasmid#103971PurposePlasmid encoding mCerulean MEDepositorInsertmCerulean ME
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCB7 alpha5
Plasmid#16041DepositorInsertalpha5 (ITGA5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEB2-DsRed-Express2
Plasmid#103999PurposePlasmid encoding DsRed-Express2DepositorInsertDsRed-Express2
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Flag-HA-Merlin
Plasmid#27104DepositorInsertMerlin (Nf2 Mouse)
UseTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
mCherry-INPP5J
Plasmid#171949PurposeExpresses INPP5J in mammalian cellsDepositorInsertINPP5J (INPP5J Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Merlin-Flag-HA
Plasmid#27103DepositorInsertMerlin (Nf2 Mouse)
UseTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2
Plasmid#128166PurposeMonomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)DepositorInsertmTagBFP2-T2A-sfGFP-iCre-ERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
GAE teo-GFP
Plasmid#83958PurposeCan be used to produce inducible SIV-derived vector. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter.DepositorInsertTetO-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAtipiTrc
Plasmid#53275PurposeContains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.DepositorInsertipi1 (IPP2 Mustard Weed)
UseTagsExpressionBacterialMutationPromoterTrcAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Myog'-2A-mCherryNLS-PGK-Purodtk
Plasmid#69547PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…ExpressionMutationSilent V205V mutation in myogenin gene to prevent…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 DN-Src
Plasmid#19965DepositorInsertSrc (SRC Chicken)
UseTagsExpressionMammalianMutationK295MPromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pIK198
Plasmid#65629Purpose"flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.DepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterU6Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK1_014
Plasmid#123677PurposeEncodes ConLS' and short flippase recognition target (FRT) site as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS'-FRT
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)
Plasmid#52897PurposeSsk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.DepositorInsertSsk1t-L-IFP-F[1]
UseTagsL-IFP-F[1]ExpressionYeastMutationPromoterGAL1Available sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone
Plasmid#219823PurposeEF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)DepositorInsertRfxCas13d
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core promoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH2.13_GagZip-MCP_Zip-P2A-eGFP
Plasmid#205528PurposeExpress HIV GagZip-MCP_Zip-P2A-eGFPDepositorInsertGagZip-MCP_Zip
UseTagsExpressionMammalianMutationNucleocapsid replaced with leucine zipperPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.14_GagZip-dp1dp6-MCP-P2A-eGFP
Plasmid#205529PurposeExpress HIV GagZip-dp1dp6-MCP-P2A-eGFPDepositorInsertGagZip-dp1dp6-MCP
UseTagsExpressionMammalianMutationNucleocapsid replaced with leucine zipper, p1 and…PromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-D34R-D87R-EGFP-6H
Plasmid#187188PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationD34R, D87RPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-L32F-L33F-EGFP-6H
Plasmid#187184PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationL32F, L33FPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90A-V91A-EGFP-6H
Plasmid#187185PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationY90A, V91APromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only