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Plasmid#171721PurposeGPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)DepositorInsertRatiometric pHLuorin2 fused to GPI anchor
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA hEZH2
Plasmid#24230PurposeMammalian expression of human EZH2 with HA tagDepositorInsertEZH2 (EZH2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAB061i
Plasmid#79226PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-HA4; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPpro1; PlacIqAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_EGFP
Plasmid#183117PurposeExpresses flag-tagged EGFP in mammalian cellsDepositorInsertEGFP
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-Smad3
Plasmid#172098PurposeExpresses fluorescently tagged human Smad3 in mammalian cells.DepositorInsertSmad 3 (Smad3 Mouse)
UseTagsmNeonGreenExpressionMammalianMutationC49S mutation in mCeruleanPromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB061i3
Plasmid#79230PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPpro1; PlacIqAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
ER-RpHLuorin2
Plasmid#171717PurposeLuminal ER expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 targeted to ER
UseTagsKDEL ER-retention signal and Signal sequence of h…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX317 GFP
Plasmid#193681PurposeConstitutive lentiviral expression of GFPDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA 3.1 Flag-rat-PTP1B
Plasmid#35988DepositorInsertprotein tyrosine phosphatase 1B (Ptpn1 Rat)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-His6
Plasmid#182386PurposeBacterial expression of Vesicle Nucleating peptideDepositorInsertVNp-His
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
800 pSG5L HA PTEN wt
Plasmid#10750DepositorInsertPTEN (PTEN Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRenilla
Plasmid#118059PurposeGBPart - Renilla Luciferase CDSDepositorInsertRenilla
UseLuciferase and Synthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSV-p65T305A
Plasmid#106454PurposeNF-kB p65, kinase site 305 mutatedDepositorInsertNF-kB p65, kinase site 305 mutated (RELA Human)
UseTagsExpressionMammalianMutationkinase site inactivatedPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFAP-Lyn-mCherry-pHluorin
Plasmid#187980PurposeMembrane targeted cytoplasmic fluorescent pH sensorDepositorInsertLyn-mcherry-pHluorin
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBRBB-eGFP
Plasmid#32549DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSS9
Plasmid#71655PurposeContains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coliDepositorInsertgfpuv
UseSynthetic BiologyTagsExpressionMutationPromoterT7A1Available sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA EED wt
Plasmid#24231PurposeMammalian expression of human EED with HA tagDepositorInsertEED (EED Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby
Plasmid#71761PurposemGFP and Synaptophysin-mRuby expression from Flp-expressing neuronsDepositorInsertsmGFP
Synaptophysin
UseAAVTagsPalmitoylation signal and mRubyExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPAmCherry-b-actin
Plasmid#31949DepositorInsertPAmCherry1
UseTagsb-actinExpressionMammalianMutationE26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…PromoterCMVAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAC-GGPPipi
Plasmid#53280PurposeContains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coliDepositorInsertcrtE, idi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only