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Plasmid#193480PurposeTurn-off fluorescent sensor for chlorideDepositorInsertGFPxm163
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP793_L2_V05_s35S_AND_GFP_pOp6_Flp_CO2_B3
Plasmid#192397PurposeTo test if a single plasmid stably transformed into Arabidopsis can switched from off to on when its AND gate unit is activated by both cell type (cortex) and chemical induction (by DEX).DepositorInsert35S::FRT-OCS-FRT::B3RT-OCS-B3RT::Turbo GFP
UseSynthetic BiologyTagsN7ExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LacZ_pHIVEGFP
Plasmid#191251PurposeExpresses LacZ in mammalian cells (used as a control)DepositorInsertLacZ
UseTagsEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
P_EGFP_AC
Plasmid#185973PurposeExpresses EGFP from PR promoter in E.coli Dh5alpha cellDepositorInsertPR-Egfp
UseSynthetic BiologyTagsExpressionMutationPromoterPRAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD_mCherry_GFP_-+
Plasmid#187390Purposearabinose-inducible expression of GFP and mCherryDepositorInsertGFP, mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D41
Plasmid#185841PurposeTesting the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D42
Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP5EC4
Plasmid#185880PurposeA plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP5EA4
Plasmid#185879PurposeA plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP5EG3
Plasmid#185878PurposeA plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (1 copy reference)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4BG6
Plasmid#185877PurposeA parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3AG4ARSd
Plasmid#185876PurposeA plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3AG4
Plasmid#185874PurposeA plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3AE4
Plasmid#185873PurposeA plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A5C
Plasmid#185872PurposeA plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1DFB
Plasmid#185871PurposeA parental plasmid for in vivo gene amplification at RPL25 locusDepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF71
Plasmid#185860PurposeTesting the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B9)
Plasmid#185858PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72
Plasmid#185857PurposeTesting the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1B
Plasmid#185856PurposeTesting the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only