Addgene

We narrowed to 4,181 results for: 027

Showing: 1501 - 1520 of 4181 results

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pMKO.1-shEsco2

Plasmid

#160957

Purpose

Esco2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Esco2 shRNA (Esco2 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shBub1

Plasmid

#160948

Purpose

Bub1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Bub1 shRNA (Bub1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAG17 IQGAP1 3'UTR

Plasmid

#14503

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

IQGAP1 3'UTR (IQGAP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pJ4M_hnRNPA2_FL_P298L

Plasmid

#139111

Purpose

Express FL hnRNPA2 P298L with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

Expression

Bacterial

Mutation

P298L

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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ECFP-Bcr NE/AA

Plasmid

#36418

Depositor

Nora Heisterkamp, John Groffen

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

breakpoint cluster region (BCR Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

N689A/ E690A

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pIS50 KIF2 3'UTR mut

Plasmid

#14494

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

KIF2 3'UTR mutant (KIF2A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
Bcr DH domain NE/AA pGEX

Plasmid

#38180

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR DH domain N689A/E690A (BCR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

BCR DH domain N689A/E690A

Promoter

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
Bcr GAP domain R1090A/pGEX 3X

Plasmid

#36406

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Bcr GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pAcBac1-GFP(39TAG)

Plasmid

#217360

Purpose

Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

Enhanced green fluorescent protein

Use

Baculoviral

Tags

Expression

Insect and Mammalian

Mutation

Y39TAG

Promoter

CMV

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-EcYtR-mCherry

Plasmid

#217362

Purpose

Expresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

E. coli tyrosine tRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2562

Plasmid

#144044

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF655 (ZNF655 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2565

Plasmid

#144131

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF655 (ZNF655 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2563

Plasmid

#142326

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF655 (ZNF655 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2561

Plasmid

#142325

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF655 (ZNF655 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-GFP-RELA K5Q

Plasmid

#196632

Purpose

Express GFP-RELA K5Q

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

GFP-RELA K5Q (RELA Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

K122Q, K123Q, K310Q, K314Q, K315Q

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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px459-Flag-Mcm2 targeting sgRNA

Plasmid

#186937

Purpose

Genomic targeting of Flag tag at Mcm2 N-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Flag-Mcm2 targeting sgRNA (Mcm2 Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Mcm2-2A mutation sgRNA

Plasmid

#186936

Purpose

sgRNA used for genetic mutation at Y81 and Y90 of Mcm2

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Mcm2-2A mutation sgRNA (Mcm2 Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdc20

Plasmid

#160954

Purpose

Cdc20 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdc20 shRNA (Cdc20 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shKif11

Plasmid

#160962

Purpose

Kif11 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Kif11 shRNA (Kif11 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shAspm

Plasmid

#160947

Purpose

Aspm shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Aspm shRNA (Aspm Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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